Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 269697..270339 Replicon chromosome
Accession NZ_CP024098
Organism Bacillus cytotoxicus strain CH_38

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag CG483_RS01540 Protein ID WP_000635963.1
Coordinates 269989..270339 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8PTJ6
Locus tag CG483_RS01535 Protein ID WP_000004571.1
Coordinates 269697..269984 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CG483_RS01515 266076..266636 - 561 WP_048721301.1 rhomboid family intramembrane serine protease -
CG483_RS01520 266727..267086 + 360 WP_011983363.1 holo-ACP synthase -
CG483_RS01525 267242..268192 + 951 WP_041810183.1 outer membrane lipoprotein carrier protein LolA -
CG483_RS01530 268314..269483 + 1170 WP_011983365.1 alanine racemase -
CG483_RS01535 269697..269984 + 288 WP_000004571.1 antitoxin EndoAI Antitoxin
CG483_RS01540 269989..270339 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
CG483_RS01545 270404..272572 + 2169 WP_048721313.1 RNA-binding transcriptional accessory protein -
CG483_RS01550 272646..272780 - 135 WP_087094358.1 cortex morphogenetic protein CmpA -
CG483_RS01555 273167..273631 + 465 WP_011983367.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T86718 WP_000635963.1 NZ_CP024098:269989-270339 [Bacillus cytotoxicus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T86718 NZ_CP024098:269989-270339 [Bacillus cytotoxicus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCGACTGTGATTGTAGCAGCGATTACTGCACAGATTCAAAAAG
CAAAATTGCCCACGCATGTGGAGATTGATGCGAAGAAGTACGGATTTGAGAGAGATTCTGTGATCTTGCTCGAGCAGATT
CGAACAATTGATAAGCAACGCTTAACGGACAAAATCACACATTTAGATGAAGTGATGATGATTCGTGTAGATGAGGCTTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10748.30 Da        Isoelectric Point: 8.9605

>AT86718 WP_000004571.1 NZ_CP024098:269697-269984 [Bacillus cytotoxicus]
VSESSVTTEIVVRLPKQVVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT86718 NZ_CP024098:269697-269984 [Bacillus cytotoxicus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAGTGGTGACGGAATTGGACGGGATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAAAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTAAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB R8PTJ6

References