Detailed information of TA system TA086714

Bioinformatically predicted

Overview

TA module

• Type: III
• Classification (family/domain): toxIN/ToxN(toxin)
• Location: 24517..25247
• Replicon: plasmid pCh39_83
• Accession: NZ_CP024097
• Organism: Bacillus cytotoxicus strain CH_39

Toxin (Protein)

• Gene name: toxN
• Uniprot ID: -
• Locus tag: CG475_RS22490
• Protein ID: WP_094374045.1
• Coordinates: 24517..25089 (-)
• Length: 191 a.a.

Antitoxin (RNA)

Gene name	toxI
Locus tag	-
Coordinates	25151..25247 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CG475_RS22475	20644..22800	-	2157	WP_063262610.1	DDE-type integrase/transposase/recombinase	-
CG475_RS22480	22797..23636	-	840	WP_063262609.1	heteromeric transposase endonuclease subunit TnsA	-
CG475_RS22485	23988..24368	-	381	WP_094374043.1	hypothetical protein	-
CG475_RS22490	24517..25089	-	573	WP_094374045.1	type III toxin-antitoxin system ToxN/AbiQ family toxin	Toxin
-	25151..25247	-	97	NuclAT_0	-	Antitoxin
-	25151..25247	-	97	NuclAT_0	-	Antitoxin
-	25151..25247	-	97	NuclAT_0	-	Antitoxin
-	25151..25247	-	97	NuclAT_0	-	Antitoxin
CG475_RS22495	25351..26373	-	1023	WP_094374047.1	DUF3991 and TOPRIM domain-containing protein	-
CG475_RS22500	26501..27052	-	552	WP_094374049.1	metal-dependent hydrolase	-
CG475_RS22505	27077..28057	-	981	WP_094374051.1	hypothetical protein	-
CG475_RS22510	28164..29933	-	1770	WP_162533114.1	hypothetical protein	-
CG475_RS22515	29930..30139	-	210	WP_094374055.1	DUF5511 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..83535	83535

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ToxN/AbiQ toxin (8-171)

Sequences

Toxin

Protein

• Length: 191 a.a.
• Molecular weight: 22516.91 Da
• Isoelectric Point: 9.5095

>T86714 WP_094374045.1 NZ_CP024097:c25089-24517 [Bacillus cytotoxicus]
MANKDNPKFYTISTKYIDYLRETDNKVPFNKDEQHSRPYVGVLEKINGHDYFVPLTSRNDKNFNSQVSVKLFDKDNPEKR
IGVLLVNNMIPVPEKECKEIDIAEKMETDPKYGNLMLKQYLFLKENMDRLSHKVEKVYKDVTVQGKPSKKQKFLKGICCD
FSTLEEKCQEYKEKNQARRIAYMRQMGRER

Nucleotide

• Length: 573 bp

>T86714 NZ_CP024097:c25089-24517 [Bacillus cytotoxicus]
TTGGCTAATAAAGATAATCCTAAATTCTATACGATTTCAACTAAATATATAGACTATCTAAGGGAAACAGATAATAAAGT
GCCCTTTAATAAAGATGAACAGCATAGTAGACCGTATGTAGGCGTATTGGAAAAAATCAATGGTCATGATTATTTTGTTC
CTTTAACATCACGTAATGATAAAAACTTTAATAGCCAGGTCAGTGTGAAATTATTCGATAAAGATAACCCTGAAAAGCGA
ATTGGAGTTTTATTAGTCAATAACATGATTCCAGTCCCAGAAAAAGAGTGTAAAGAAATTGATATAGCAGAAAAAATGGA
GACAGATCCTAAGTATGGTAATTTGATGTTAAAACAGTATTTGTTTTTAAAAGAGAATATGGATCGACTTTCTCATAAAG
TGGAAAAAGTATATAAAGATGTAACTGTACAAGGAAAGCCATCAAAAAAACAGAAATTTCTAAAGGGAATTTGCTGTGAC
TTCTCAACATTAGAAGAAAAGTGCCAGGAATATAAAGAGAAGAATCAAGCTAGAAGAATAGCATATATGCGTCAAATGGG
GCGTGAAAGATAA

Antitoxin

Nucleotide

• Length: 97 bp

>AT86714 NZ_CP024097:c25247-25151 [Bacillus cytotoxicus]
GTAGCCTTACTTTAGTAGGCGCGAAAAAATTGGTGTAACTCTACCGTAGTGAGTACCAAAAAATTGGTGTAGCCTTACCG
TAGTAGGCACAAAAAAA

Structures

Antitoxin