Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4133029..4133526 Replicon chromosome
Accession NZ_CP024090
Organism Escherichia coli strain WG5

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag CSR56_RS21090 Protein ID WP_001173343.1
Coordinates 4133029..4133202 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag CSR56_RS21095 Protein ID WP_000398619.1
Coordinates 4133230..4133526 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSR56_RS21065 4128359..4128934 + 576 WP_001188520.1 transcriptional regulator -
CSR56_RS21080 4130602..4131156 + 555 Protein_3862 dipeptide/tripeptide permease DtpC -
CSR56_RS21085 4131393..4132910 + 1518 WP_001295074.1 lysine--tRNA ligase -
CSR56_RS21090 4133029..4133202 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
CSR56_RS21095 4133230..4133526 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
CSR56_RS21105 4133753..4134025 - 273 WP_000405647.1 N-acetyltransferase -
CSR56_RS21110 4134037..4134267 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
CSR56_RS21115 4134448..4136079 + 1632 WP_047402662.1 sensor histidine kinase -
CSR56_RS21120 4136076..4136795 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T86711 WP_001173343.1 NZ_CP024090:c4133202-4133029 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T86711 NZ_CP024090:c4133202-4133029 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT86711 WP_000398619.1 NZ_CP024090:c4133526-4133230 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT86711 NZ_CP024090:c4133526-4133230 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References