Detailed information of TA system TA086685

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 992204..992712
• Replicon: chromosome
• Accession: NZ_CP024086
• Organism: Pseudomonas putida strain E46

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: CR512_RS04385
• Protein ID: WP_099428364.1
• Coordinates: 992204..992419 (+)
• Length: 72 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A285YW69
• Locus tag: CR512_RS04390
• Protein ID: WP_027595525.1
• Coordinates: 992416..992712 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR512_RS04360	987848..988702	+	855	WP_099428359.1	putative selenate ABC transporter substrate-binding protein	-
CR512_RS04365	988699..989514	+	816	WP_099428360.1	ATP-binding cassette domain-containing protein	-
CR512_RS04370	989508..990335	+	828	WP_099428361.1	ABC transporter permease subunit	-
CR512_RS04375	990332..991099	+	768	WP_099428362.1	phosphonate ABC transporter, permease protein PhnE	-
CR512_RS04380	991129..992019	-	891	WP_099428363.1	effector protein	-
CR512_RS04385	992204..992419	+	216	WP_099428364.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CR512_RS04390	992416..992712	+	297	WP_027595525.1	putative addiction module antidote protein	Antitoxin
CR512_RS04395	992723..993202	-	480	WP_099428365.1	GNAT family N-acetyltransferase	-
CR512_RS04400	993296..993943	-	648	WP_099430866.1	serine protease	-
CR512_RS04410	994504..994722	-	219	WP_085272636.1	hypothetical protein	-
CR512_RS04415	994818..995720	-	903	WP_099428366.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
CR512_RS04420	995707..996261	-	555	WP_099428367.1	hypothetical protein	-
CR512_RS04430	996639..997688	+	1050	WP_027595528.1	tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-69)

Antitoxin

• Uncharacterized protein HI1420 (5-91)

Sequences

Toxin

Protein

• Length: 72 a.a.
• Molecular weight: 7814.23 Da
• Isoelectric Point: 10.9229

>T86685 WP_099428364.1 NZ_CP024086:992204-992419 [Pseudomonas putida]
MNVSKRIQKVETLATTKALGGVSELRINVSAGYRVYFTRKGAKVIFLLVGGDKSSQTADILKARKLVEEMK

Nucleotide

• Length: 216 bp

>T86685 NZ_CP024086:992204-992419 [Pseudomonas putida]
ATGAATGTATCCAAAAGGATACAGAAAGTGGAAACCTTGGCGACTACAAAAGCGTTGGGGGGTGTAAGTGAGCTTCGCAT
CAACGTTTCTGCAGGTTATCGAGTCTATTTCACGCGTAAGGGAGCCAAAGTGATTTTTCTGCTGGTGGGCGGAGACAAAT
CTTCCCAGACCGCCGATATCCTCAAAGCGAGAAAGCTGGTGGAGGAGATGAAATGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10592.09 Da
• Isoelectric Point: 5.5802

>AT86685 WP_027595525.1 NZ_CP024086:992416-992712 [Pseudomonas putida]
MSEKLIPFDIADLLDSDAAISEYLSQVLSDGDTDEIIRAIGHIARARGMTQIAAQSGLGRESLYKALSPGAKPRFDTVLK
VIRALGVELFVQPHTSSH

Nucleotide

• Length: 297 bp

>AT86685 NZ_CP024086:992416-992712 [Pseudomonas putida]
ATGAGCGAAAAATTGATCCCTTTTGATATCGCCGACTTGCTTGACAGTGATGCAGCAATCAGCGAATACCTCAGCCAGGT
GCTATCCGATGGCGATACAGATGAGATTATCCGGGCCATCGGTCATATCGCTCGGGCACGAGGAATGACACAAATCGCTG
CACAGAGTGGGTTAGGTCGAGAAAGTCTCTATAAAGCACTTAGCCCAGGCGCTAAGCCCCGCTTTGATACAGTGCTTAAA
GTCATACGTGCCCTAGGCGTGGAGCTTTTTGTTCAGCCCCATACGTCGTCGCATTAG

Structures

Antitoxin

• AlphaFold DB: A0A285YW69