Detailed information of TA system TA086682

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 2175182..2175698
• Replicon: chromosome
• Accession: NZ_CP024085
• Organism: Pseudomonas putida strain E41

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CR511_RS09865
• Protein ID: WP_099453570.1
• Coordinates: 2175417..2175698 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A285Z3X6
• Locus tag: CR511_RS09860
• Protein ID: WP_027593796.1
• Coordinates: 2175182..2175427 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR511_RS09840	2171669..2172403	+	735	WP_099453568.1	hypothetical protein	-
CR511_RS09845	2172504..2173648	+	1145	Protein_1891	IS3 family transposase	-
CR511_RS09850	2173881..2174369	+	489	WP_099453569.1	transposase	-
CR511_RS09855	2174471..2175013	-	543	WP_099455818.1	WYL domain-containing protein	-
CR511_RS09860	2175182..2175427	+	246	WP_027593796.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CR511_RS09865	2175417..2175698	+	282	WP_099453570.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CR511_RS09870	2175756..2176676	-	921	WP_099453571.1	LysR family transcriptional regulator	-
CR511_RS09875	2176844..2178148	+	1305	WP_099453572.1	MHS family MFS transporter	-
CR511_RS09880	2178179..2179420	+	1242	WP_099453573.1	Zn-dependent hydrolase	-
CR511_RS09885	2179420..2180442	+	1023	WP_099453574.1	histone deacetylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-82)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (10-75)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10907.79 Da
• Isoelectric Point: 10.8716

>T86682 WP_099453570.1 NZ_CP024085:2175417-2175698 [Pseudomonas putida]
MTYKLEFVPSAFKEWGKLGHTLREQIKKKLGERMQAPRVQADALRELPNHYKIKFKASGYRLVYRVEDERVVVVVVSVGK
RERSAVYQSAKKR

Nucleotide

• Length: 282 bp

>T86682 NZ_CP024085:2175417-2175698 [Pseudomonas putida]
ATGACTTATAAGCTTGAGTTCGTCCCTTCAGCGTTCAAGGAGTGGGGCAAACTGGGCCATACGTTGCGTGAGCAGATCAA
GAAGAAGCTTGGCGAACGTATGCAGGCACCGAGGGTGCAGGCCGATGCTCTGCGGGAGCTGCCTAATCACTACAAAATAA
AGTTCAAGGCTTCAGGCTACCGCCTCGTCTACCGGGTCGAAGATGAGCGGGTCGTTGTTGTGGTAGTTTCAGTGGGCAAG
CGTGAACGCAGCGCGGTTTATCAGTCAGCCAAAAAGCGCTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8779.20 Da
• Isoelectric Point: 4.2103

>AT86682 WP_027593796.1 NZ_CP024085:2175182-2175427 [Pseudomonas putida]
MQNIFADVAVSVSELKKNPTAVMSGAAGMPIAVLNHNRVMGYMVPAELYEAMMEKLEDIELSELAKSRLGEQGIPVSLDD
L

Nucleotide

• Length: 246 bp

>AT86682 NZ_CP024085:2175182-2175427 [Pseudomonas putida]
ATGCAGAACATTTTCGCTGACGTAGCGGTAAGCGTTTCAGAGCTAAAAAAGAACCCAACTGCAGTAATGTCTGGCGCCGC
AGGCATGCCCATTGCCGTACTGAATCACAATCGTGTGATGGGTTACATGGTGCCGGCCGAACTGTATGAAGCCATGATGG
AAAAATTGGAAGACATCGAGCTAAGCGAGCTGGCCAAGTCCCGTCTTGGTGAGCAAGGTATACCGGTGTCTTTGGATGAC
TTATAA

Structures

Antitoxin

• AlphaFold DB: A0A285Z3X6