Detailed information of TA system TA086679

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 1002884..1003347
• Replicon: chromosome
• Accession: NZ_CP024085
• Organism: Pseudomonas putida strain E41

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: CR511_RS04475
• Protein ID: WP_099452955.1
• Coordinates: 1002884..1003054 (+)
• Length: 57 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A285YW69
• Locus tag: CR511_RS04480
• Protein ID: WP_027595525.1
• Coordinates: 1003051..1003347 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR511_RS04450	998536..999390	+	855	WP_027595519.1	putative selenate ABC transporter substrate-binding protein	-
CR511_RS04455	999387..1000202	+	816	WP_099452952.1	ATP-binding cassette domain-containing protein	-
CR511_RS04460	1000196..1001023	+	828	WP_099452953.1	ABC transporter permease	-
CR511_RS04465	1001020..1001787	+	768	WP_099452954.1	phosphonate ABC transporter, permease protein PhnE	-
CR511_RS04470	1001817..1002707	-	891	WP_027595523.1	effector protein	-
CR511_RS04475	1002884..1003054	+	171	WP_099452955.1	addiction module killer protein	Toxin
CR511_RS04480	1003051..1003347	+	297	WP_027595525.1	putative addiction module antidote protein	Antitoxin
CR511_RS04485	1003358..1003837	-	480	WP_085272634.1	GNAT family N-acetyltransferase	-
CR511_RS04490	1003931..1004578	-	648	WP_099455787.1	serine protease	-
CR511_RS04495	1004923..1006254	+	1332	WP_099452956.1	Fic family protein	-
CR511_RS04500	1006316..1006738	+	423	WP_099452957.1	VOC family protein	-
CR511_RS04510	1007186..1008235	+	1050	WP_027595528.1	tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (5-54)

Antitoxin

• Uncharacterized protein HI1420 (5-91)

Sequences

Toxin

Protein

• Length: 57 a.a.
• Molecular weight: 6244.34 Da
• Isoelectric Point: 9.7236

>T86679 WP_099452955.1 NZ_CP024085:1002884-1003054 [Pseudomonas putida]
ISHECGVSELRIDVSAGYRVYFMRKGAKVIFLLVGGDKSSQTADILKARKLVEEMK

Nucleotide

• Length: 171 bp

>T86679 NZ_CP024085:1002884-1003054 [Pseudomonas putida]
ATCTCGCATGAATGCGGTGTAAGTGAGCTTCGCATCGACGTTTCTGCAGGTTATCGAGTCTATTTCATGCGTAAGGGAGC
CAAAGTGATTTTTCTGCTGGTGGGCGGAGACAAATCTTCCCAGACCGCCGATATCCTCAAAGCGAGAAAGCTGGTGGAGG
AAATGAAATGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10592.09 Da
• Isoelectric Point: 5.5802

>AT86679 WP_027595525.1 NZ_CP024085:1003051-1003347 [Pseudomonas putida]
MSEKLIPFDIADLLDSDAAISEYLSQVLSDGDTDEIIRAIGHIARARGMTQIAAQSGLGRESLYKALSPGAKPRFDTVLK
VIRALGVELFVQPHTSSH

Nucleotide

• Length: 297 bp

>AT86679 NZ_CP024085:1003051-1003347 [Pseudomonas putida]
ATGAGCGAAAAATTGATCCCTTTTGATATCGCCGACTTGCTTGACAGTGATGCAGCAATCAGCGAATACCTCAGCCAGGT
GCTATCCGATGGCGATACAGATGAGATTATCCGGGCCATCGGTCATATCGCTCGGGCACGGGGAATGACACAAATCGCTG
CACAGAGTGGGTTAGGTCGAGAAAGTCTCTATAAAGCACTTAGCCCAGGCGCTAAGCCCCGCTTTGATACAGTGCTTAAA
GTCATACGTGCCCTAGGCGTGGAGCTTTTTGTTCAGCCCCATACGTCGTCGCATTAG

Structures

Antitoxin

• AlphaFold DB: A0A285YW69