86677

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /GNAT-DUF1778
Location 370617..371383 Replicon chromosome
Accession NZ_CP024083
Organism Vibrio cholerae strain FORC_073

Toxin (Protein)


Gene name - Uniprot ID Q9K2P7
Locus tag FORC73_RS16345 Protein ID WP_000982260.1
Coordinates 370617..371114 (-) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9K2J6
Locus tag FORC73_RS16350 Protein ID WP_000246253.1
Coordinates 371111..371383 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC73_RS16315 365960..366112 + 153 WP_032469020.1 DUF645 family protein -
FORC73_RS16320 366327..367331 + 1005 WP_000964935.1 LD-carboxypeptidase -
FORC73_RS16325 367469..368122 + 654 WP_113612641.1 hypothetical protein -
FORC73_RS16330 368157..368794 - 638 Protein_354 IS30 family transposase -
FORC73_RS16335 368862..370039 + 1178 WP_162906056.1 IS3 family transposase -
FORC73_RS16340 370047..370376 - 330 Protein_356 IS30 family transposase -
FORC73_RS16345 370617..371114 - 498 WP_000982260.1 GNAT family N-acetyltransferase Toxin
FORC73_RS16350 371111..371383 - 273 WP_000246253.1 DUF1778 domain-containing protein Antitoxin
FORC73_RS16355 371596..371895 - 300 WP_001247271.1 type II toxin-antitoxin system RelE/ParE family toxin -
FORC73_RS16360 371892..372134 - 243 WP_001107716.1 type II toxin-antitoxin system ParD family antitoxin -
FORC73_RS16365 372723..375509 + 2787 WP_001966133.1 alkaline phosphatase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 308518..372134 63616
- inside Integron - - 309195..371895 62700


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(7-86)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18527.18 Da        Isoelectric Point: 8.7753

>T86677 WP_000982260.1 NZ_CP024083:c371114-370617 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Download         Length: 498 bp

>T86677 NZ_CP024083:c371114-370617 [Vibrio cholerae]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATTGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGCGTAGCGTTCCCAGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9683.22 Da        Isoelectric Point: 6.2988

>AT86677 WP_000246253.1 NZ_CP024083:c371383-371111 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Download         Length: 273 bp

>AT86677 NZ_CP024083:c371383-371111 [Vibrio cholerae]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCGGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0KGB1

Antitoxin

Source ID Structure
AlphaFold DB A0A0F2IC79

References