Detailed information of TA system TA086676

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 362414..362961
• Replicon: chromosome
• Accession: NZ_CP024083
• Organism: Vibrio cholerae strain FORC_073

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: FORC73_RS16280
• Protein ID: WP_000818171.1
• Coordinates: 362659..362961 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q9KM93
• Locus tag: FORC73_RS16275
• Protein ID: WP_000861987.1
• Coordinates: 362414..362671 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC73_RS16235	357816..358334	+	519	WP_000698780.1	GNAT family N-acetyltransferase	-
FORC73_RS16240	358479..359423	+	945	WP_086584918.1	restriction endonuclease	-
FORC73_RS16245	359575..360072	+	498	WP_000259910.1	GNAT family N-acetyltransferase	-
FORC73_RS16255	360192..360473	+	282	WP_119783490.1	DUF1289 domain-containing protein	-
FORC73_RS16265	360852..361784	+	933	WP_001267735.1	hypothetical protein	-
FORC73_RS16270	361768..362196	+	429	WP_119783491.1	hypothetical protein	-
FORC73_RS16275	362414..362671	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FORC73_RS16280	362659..362961	+	303	WP_000818171.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FORC73_RS16285	363431..363556	+	126	WP_001933211.1	DUF3265 domain-containing protein	-
FORC73_RS16295	363788..363893	+	106	Protein_348	DUF645 family protein	-
FORC73_RS16300	364095..364328	+	234	WP_148535500.1	hypothetical protein	-
FORC73_RS16305	364428..365468	+	1041	WP_032475915.1	IS481 family transposase	-
FORC73_RS16315	365960..366112	+	153	WP_032469020.1	DUF645 family protein	-
FORC73_RS16320	366327..367331	+	1005	WP_000964935.1	LD-carboxypeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	308518..372134	63616
	-	inside	Integron	-	-	309195..371895	62700

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11709.57 Da
• Isoelectric Point: 4.8838

>T86676 WP_000818171.1 NZ_CP024083:362659-362961 [Vibrio cholerae]
MAEIVWTEPALSDLNDIAEYIALENVVAAKQLVQTIFTKVERLADFPESGRIPPELEHLNYREVVVNPCRVFYKYDDANV
RILFVMRAERDLRRLMLTKQ

Nucleotide

• Length: 303 bp

>T86676 NZ_CP024083:362659-362961 [Vibrio cholerae]
ATGGCTGAAATAGTTTGGACGGAACCAGCTCTATCCGATCTAAATGATATTGCTGAATACATAGCGCTTGAAAATGTCGT
AGCTGCTAAACAACTGGTGCAAACTATTTTTACAAAAGTTGAACGTTTGGCCGATTTTCCAGAGTCTGGACGCATTCCAC
CAGAACTAGAGCACCTCAATTATCGTGAAGTCGTTGTAAATCCGTGCCGTGTTTTCTACAAGTATGATGATGCAAATGTT
CGTATTCTTTTTGTTATGCGTGCGGAGCGAGATTTGCGTCGGTTAATGCTTACGAAACAGTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9647.15 Da
• Isoelectric Point: 7.3178

>AT86676 WP_000861987.1 NZ_CP024083:362414-362671 [Vibrio cholerae]
MKVELVTSLKRQATKILADLHDTKEPVLITEHGKPSAYLIDVDDYEFMQNRLAILEGIARGERALADGKVVSHQDAKDRM
SKWLK

Nucleotide

• Length: 258 bp

>AT86676 NZ_CP024083:362414-362671 [Vibrio cholerae]
ATGAAAGTAGAACTTGTAACTTCACTAAAACGGCAGGCCACTAAGATCCTTGCCGATCTTCATGATACTAAAGAGCCAGT
ATTGATAACTGAGCATGGAAAGCCGTCAGCTTATCTTATTGATGTAGATGATTATGAATTTATGCAAAATCGTTTAGCAA
TCCTCGAAGGAATTGCTCGTGGTGAGCGAGCTCTAGCGGATGGCAAAGTAGTCAGCCATCAAGATGCTAAGGACAGAATG
TCAAAATGGCTGAAATAG

Structures

Antitoxin

• AlphaFold DB: A0A1T4SLM9