Detailed information of TA system TA086671

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 329421..329980
• Replicon: chromosome
• Accession: NZ_CP024083
• Organism: Vibrio cholerae strain FORC_073

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D7HHK2
• Locus tag: FORC73_RS15840
• Protein ID: WP_000578471.1
• Coordinates: 329421..329699 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q9L991
• Locus tag: FORC73_RS15845
• Protein ID: WP_000381183.1
• Coordinates: 329696..329980 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC73_RS19345	324515..324604	+	90	Protein_279	GNAT family N-acetyltransferase	-
FORC73_RS15790	325032..325790	+	759	WP_119783474.1	hypothetical protein	-
FORC73_RS15795	325967..326320	+	354	WP_174232559.1	nuclear transport factor 2 family protein	-
FORC73_RS15805	326437..326754	-	318	WP_119783476.1	CcdB family protein	-
FORC73_RS15810	326754..326999	-	246	WP_001260797.1	type II toxin-antitoxin system CcdA family antitoxin	-
FORC73_RS15815	327230..327718	+	489	WP_114736409.1	hypothetical protein	-
FORC73_RS15830	328278..328868	+	591	WP_000589662.1	DUF1294 domain-containing protein	-
FORC73_RS15840	329421..329699	-	279	WP_000578471.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
FORC73_RS15845	329696..329980	-	285	WP_000381183.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
FORC73_RS15850	330186..330725	+	540	WP_000884557.1	hydrolase	-
FORC73_RS15860	330893..331717	+	825	WP_153734389.1	hypothetical protein	-
FORC73_RS15865	331714..331836	+	123	WP_119783597.1	DUF3265 domain-containing protein	-
FORC73_RS15870	332024..332203	+	180	WP_000887876.1	DUF645 family protein	-
FORC73_RS15875	332352..332714	+	363	WP_161418255.1	DUF4144 domain-containing protein	-
FORC73_RS15890	333034..333204	+	171	WP_001928536.1	DUF645 family protein	-
FORC73_RS15900	333405..333701	+	297	WP_000617998.1	Dabb family protein	-
FORC73_RS15905	333953..334507	+	555	WP_162923449.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	308518..372134	63616
	-	inside	Integron	-	-	309195..371895	62700

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-70)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10838.56 Da
• Isoelectric Point: 4.6794

>T86671 WP_000578471.1 NZ_CP024083:c329699-329421 [Vibrio cholerae]
MIFWEEASLNDREKIFEFLYDFNPAAAEKTDELIEAKVENLLEQPLMGVQRDGIRGRLLIIPEISMIVSYWVDGSKIRIM
RVLHQKQKFPND

Nucleotide

• Length: 279 bp

>T86671 NZ_CP024083:c329699-329421 [Vibrio cholerae]
ATGATTTTCTGGGAAGAAGCATCTCTCAATGATCGTGAGAAAATTTTCGAATTTCTCTACGACTTTAACCCAGCAGCCGC
TGAAAAAACGGATGAGCTCATAGAAGCCAAAGTTGAAAATTTGCTTGAGCAACCACTAATGGGTGTTCAGCGTGATGGCA
TTAGAGGCAGATTACTTATTATCCCTGAGATATCAATGATTGTTTCGTATTGGGTTGATGGTTCTAAAATTCGAATAATG
CGAGTACTACATCAGAAACAAAAATTTCCAAACGACTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10901.25 Da
• Isoelectric Point: 8.0775

>AT86671 WP_000381183.1 NZ_CP024083:c329980-329696 [Vibrio cholerae]
MDTRIQFRVDEETKRLAQQMAESQGRTLSDACRELTEQLAEQQRKALSHDAWLTEQVNQAFEKFDSGKAVFIEHDIAKAR
MAERKAKIRNRGHA

Nucleotide

• Length: 285 bp

>AT86671 NZ_CP024083:c329980-329696 [Vibrio cholerae]
ATGGATACTAGAATTCAATTTCGTGTAGACGAAGAAACAAAACGTTTAGCTCAACAAATGGCTGAAAGCCAAGGTCGAAC
TCTTAGCGATGCTTGTCGTGAACTCACTGAACAATTAGCTGAGCAGCAACGCAAGGCATTATCTCACGATGCATGGCTAA
CTGAACAAGTGAATCAAGCATTTGAGAAGTTCGACTCAGGAAAAGCAGTATTCATTGAACATGACATCGCCAAAGCACGA
ATGGCTGAACGTAAAGCTAAAATCCGAAATCGAGGCCACGCATGA

Structures

Toxin

• AlphaFold DB: A0A271VKS2

Antitoxin

• AlphaFold DB: A0A067BL33