Detailed information of TA system TA086652

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 3301623..3302101
• Replicon: chromosome
• Accession: NZ_CP024056
• Organism: Escherichia coli strain SMN197SH3

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: CRT55_RS19265
• Protein ID: WP_001303876.1
• Coordinates: 3301814..3302101 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: CRT55_RS19260
• Protein ID: WP_000536233.1
• Coordinates: 3301623..3301814 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRT55_RS19210	3296659..3296937	+	279	WP_074433979.1	hypothetical protein	-
CRT55_RS19215	3296939..3297988	+	1050	WP_001265133.1	DUF968 domain-containing protein	-
CRT55_RS19220	3298001..3298372	+	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
CRT55_RS19225	3298362..3298733	+	372	WP_000090265.1	antitermination protein	-
CRT55_RS19230	3298885..3299703	+	819	WP_000265267.1	CPBP family intramembrane metalloprotease	-
CRT55_RS19235	3299990..3300187	+	198	WP_000917737.1	hypothetical protein	-
CRT55_RS30435	3300322..3300747	+	426	WP_176465538.1	hypothetical protein	-
CRT55_RS19245	3300734..3300829	-	96	Protein_3384	Rha family transcriptional regulator	-
CRT55_RS19250	3300813..3301085	-	273	WP_000887453.1	helix-turn-helix domain-containing protein	-
CRT55_RS19255	3301194..3301595	+	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
CRT55_RS19260	3301623..3301814	+	192	WP_000536233.1	hypothetical protein	Antitoxin
CRT55_RS19265	3301814..3302101	+	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CRT55_RS19270	3302378..3302533	+	156	WP_000379575.1	DUF1391 family protein	-
CRT55_RS19280	3302675..3303064	+	390	WP_032205089.1	hypothetical protein	-
CRT55_RS19285	3303251..3303436	-	186	WP_032205087.1	hypothetical protein	-
CRT55_RS19295	3304010..3304198	+	189	WP_000413705.1	cell division inhibition protein DicB	-
CRT55_RS19300	3304195..3304386	+	192	WP_001098307.1	DUF1482 family protein	-
CRT55_RS19305	3304480..3306915	+	2436	WP_101975670.1	exonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T86652 WP_001303876.1 NZ_CP024056:3301814-3302101 [Escherichia coli]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T86652 NZ_CP024056:3301814-3302101 [Escherichia coli]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT86652 WP_000536233.1 NZ_CP024056:3301623-3301814 [Escherichia coli]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT86652 NZ_CP024056:3301623-3301814 [Escherichia coli]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA