Detailed information of TA system TA086641

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1322851..1323476
• Replicon: chromosome
• Accession: NZ_CP024056
• Organism: Escherichia coli strain SMN197SH3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CRT55_RS07905
• Protein ID: WP_000911330.1
• Coordinates: 1323078..1323476 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: CRT55_RS07900
• Protein ID: WP_000450524.1
• Coordinates: 1322851..1323078 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRT55_RS07875	1318652..1319122	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
CRT55_RS07880	1319122..1319694	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
CRT55_RS07885	1319840..1320718	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
CRT55_RS07890	1320735..1321769	+	1035	WP_062882059.1	outer membrane protein assembly factor BamC	-
CRT55_RS07895	1321982..1322695	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
CRT55_RS07900	1322851..1323078	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
CRT55_RS07905	1323078..1323476	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CRT55_RS07910	1323623..1324486	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
CRT55_RS07915	1324501..1326516	+	2016	WP_062882060.1	tRNA cytosine(34) acetyltransferase TmcA	-
CRT55_RS07920	1326590..1327192	+	603	Protein_1285	esterase	-
CRT55_RS07925	1327302..1327502	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T86641 WP_000911330.1 NZ_CP024056:1323078-1323476 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T86641 NZ_CP024056:1323078-1323476 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT86641 WP_000450524.1 NZ_CP024056:1322851-1323078 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT86641 NZ_CP024056:1322851-1323078 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33