Detailed information of TA system TA086629

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 68635..69194
• Replicon: plasmid pO177A3
• Accession: NZ_CP024055
• Organism: Escherichia coli strain SMN197SH3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PMX5
• Locus tag: CRT55_RS00765
• Protein ID: WP_000421257.1
• Coordinates: 68635..68910 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CRT55_RS00770
• Protein ID: WP_062881718.1
• Coordinates: 68910..69194 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRT55_RS00760	67205..68418	+	1214	WP_162829200.1	IS3 family transposase	-
CRT55_RS00765	68635..68910	-	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CRT55_RS00770	68910..69194	-	285	WP_062881718.1	ribbon-helix-helix domain-containing protein	Antitoxin
CRT55_RS00780	70106..70963	-	858	WP_096851680.1	incFII family plasmid replication initiator RepA	-
CRT55_RS00785	70956..71438	-	483	WP_001523473.1	hypothetical protein	-
CRT55_RS00790	71431..71505	-	75	WP_001442103.1	RepA leader peptide Tap	-
CRT55_RS00800	71737..71994	-	258	WP_000083845.1	replication regulatory protein RepA	-
CRT55_RS00805	72278..72484	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	-
-	72471..72532	+	62	NuclAT_0	-	-
-	72471..72532	+	62	NuclAT_0	-	-
-	72471..72532	+	62	NuclAT_0	-	-
-	72471..72532	+	62	NuclAT_0	-	-
CRT55_RS30290	72788..72862	-	75	Protein_74	endonuclease	-
CRT55_RS00815	73108..73320	-	213	WP_001299730.1	ANR family transcriptional regulator	-
CRT55_RS00820	73456..74016	-	561	WP_062881716.1	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hlyD / espP / hlyC / hlyA / hlyB	1..88839	88839

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T86629 WP_000421257.1 NZ_CP024055:c68910-68635 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T86629 NZ_CP024055:c68910-68635 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10460.87 Da
• Isoelectric Point: 5.6471

>AT86629 WP_062881718.1 NZ_CP024055:c69194-68910 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLDALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT86629 NZ_CP024055:c69194-68910 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGATGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAAGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: S1PMX5