Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 29552..30144 Replicon plasmid pMR0617mcr
Accession NZ_CP024041
Organism Klebsiella pneumoniae strain QS17-0029

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag CR230_RS28730 Protein ID WP_000520549.1
Coordinates 29896..30144 (-) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag CR230_RS28725 Protein ID WP_000681613.1
Coordinates 29552..29899 (-) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR230_RS28690 25557..25829 - 273 WP_001328551.1 TrbC/VirB2 family protein -
CR230_RS28695 25897..26517 - 621 WP_000157549.1 lytic transglycosylase domain-containing protein -
CR230_RS28700 26873..27088 - 216 WP_000760375.1 EexN family lipoprotein -
CR230_RS28705 27085..27378 - 294 WP_000134733.1 LuxR family transcriptional regulator -
CR230_RS28710 27455..28684 - 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
CR230_RS28715 28688..29191 - 504 WP_000781813.1 hypothetical protein -
CR230_RS28725 29552..29899 - 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CR230_RS28730 29896..30144 - 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
CR230_RS28735 30449..30799 - 351 WP_000854259.1 hypothetical protein -
CR230_RS28740 30890..31189 - 300 WP_000650309.1 hypothetical protein -
CR230_RS28750 31643..31870 - 228 WP_000801440.1 hypothetical protein -
CR230_RS28755 31857..32108 - 252 WP_001230707.1 hypothetical protein -
CR230_RS28760 32105..32647 - 543 WP_001083906.1 hypothetical protein -
CR230_RS29120 32752..33267 - 516 WP_001025395.1 J domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-1.1 - 1..33858 33858


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T86622 WP_000520549.1 NZ_CP024041:c30144-29896 [Klebsiella pneumoniae]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T86622 NZ_CP024041:c30144-29896 [Klebsiella pneumoniae]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT86622 WP_000681613.1 NZ_CP024041:c29899-29552 [Klebsiella pneumoniae]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT86622 NZ_CP024041:c29899-29552 [Klebsiella pneumoniae]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References