Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 31559..32118 Replicon plasmid pMR0617ndm
Accession NZ_CP024039
Organism Klebsiella pneumoniae strain QS17-0029

Toxin (Protein)


Gene name relE Uniprot ID V0SVC8
Locus tag CR230_RS27060 Protein ID WP_000421272.1
Coordinates 31559..31834 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag CR230_RS27065 Protein ID WP_001387467.1
Coordinates 31834..32118 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR230_RS27020 27609..28349 - 741 WP_001066942.1 tyrosine-type recombinase/integrase -
CR230_RS27025 28470..28652 - 183 WP_042065278.1 hypothetical protein -
CR230_RS27035 28995..29302 - 308 Protein_28 type II toxin-antitoxin system ParD family antitoxin -
CR230_RS27045 29795..30964 + 1170 WP_001072355.1 hypothetical protein -
CR230_RS27050 30997..31125 + 129 Protein_30 IS481 family transposase -
CR230_RS27055 31160..31453 - 294 WP_001105060.1 hypothetical protein -
CR230_RS27060 31559..31834 - 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CR230_RS27065 31834..32118 - 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
CR230_RS27075 32772..33469 + 698 Protein_34 IS1 family transposase -
CR230_RS27080 33484..34647 + 1164 Protein_35 IS4-like element ISVsa5 family transposase -
CR230_RS27085 34657..35028 - 372 WP_035689358.1 helix-turn-helix domain-containing protein -
CR230_RS27090 35161..35754 + 594 WP_000428546.1 tetracyline resistance-associated transcriptional repressor TetC -
CR230_RS27095 35867..37072 - 1206 WP_001089068.1 tetracycline efflux MFS transporter Tet(B) -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(B) / dfrA12 / aadA2 / qacE / sul1 / blaNDM-1 - 1..125285 125285


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 10.0871

>T86615 WP_000421272.1 NZ_CP024039:c31834-31559 [Klebsiella pneumoniae]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T86615 NZ_CP024039:c31834-31559 [Klebsiella pneumoniae]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT86615 WP_001387467.1 NZ_CP024039:c32118-31834 [Klebsiella pneumoniae]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT86615 NZ_CP024039:c32118-31834 [Klebsiella pneumoniae]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References