Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 17643..18244 Replicon plasmid pMR0617ndm
Accession NZ_CP024039
Organism Klebsiella pneumoniae strain QS17-0029

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag CR230_RS26950 Protein ID WP_001216034.1
Coordinates 17864..18244 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag CR230_RS26945 Protein ID WP_001190712.1
Coordinates 17643..17864 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR230_RS26920 13859..15130 - 1272 WP_048266692.1 restriction endonuclease subunit S -
CR230_RS26925 15127..15417 - 291 Protein_10 N-6 DNA methylase -
CR230_RS26935 15473..16170 + 698 Protein_11 IS1 family transposase -
CR230_RS26940 16165..17460 - 1296 Protein_12 type I restriction-modification system subunit M -
CR230_RS26945 17643..17864 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CR230_RS26950 17864..18244 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
CR230_RS26955 18249..18428 + 180 WP_001513661.1 hypothetical protein -
CR230_RS26960 18456..18815 + 360 WP_001513660.1 hypothetical protein -
CR230_RS26965 18739..19152 + 414 Protein_17 DDE-type integrase/transposase/recombinase -
CR230_RS26970 19102..19419 - 318 WP_001513659.1 hypothetical protein -
CR230_RS26975 19647..20663 - 1017 WP_012372828.1 IS5-like element IS5 family transposase -
CR230_RS26980 20871..22274 + 1404 WP_001373486.1 S-methylmethionine permease -
CR230_RS26985 22261..23193 + 933 WP_000081352.1 homocysteine S-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(B) / dfrA12 / aadA2 / qacE / sul1 / blaNDM-1 - 1..125285 125285


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T86614 WP_001216034.1 NZ_CP024039:17864-18244 [Klebsiella pneumoniae]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T86614 NZ_CP024039:17864-18244 [Klebsiella pneumoniae]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTAGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT86614 WP_001190712.1 NZ_CP024039:17643-17864 [Klebsiella pneumoniae]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT86614 NZ_CP024039:17643-17864 [Klebsiella pneumoniae]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References