Detailed information of TA system TA086595

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 200568..201204
• Replicon: chromosome
• Accession: NZ_CP024035
• Organism: Priestia aryabhattai strain K13

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D5DWT4
• Locus tag: CR091_RS01140
• Protein ID: WP_013055004.1
• Coordinates: 200854..201204 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: D5DWT3
• Locus tag: CR091_RS01135
• Protein ID: WP_013055003.1
• Coordinates: 200568..200849 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR091_RS01110	195934..196905	+	972	WP_033580801.1	UV DNA damage repair endonuclease UvsE	-
CR091_RS01115	196914..197516	-	603	WP_028411882.1	rhomboid family intramembrane serine protease	-
CR091_RS01120	197581..197946	+	366	WP_028411881.1	holo-ACP synthase	-
CR091_RS01125	198005..199063	+	1059	WP_033580802.1	outer membrane lipoprotein carrier protein LolA	-
CR091_RS01130	199177..200367	+	1191	WP_034327253.1	alanine racemase	-
CR091_RS01135	200568..200849	+	282	WP_013055003.1	hypothetical protein	Antitoxin
CR091_RS01140	200854..201204	+	351	WP_013055004.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
CR091_RS01145	201362..202198	+	837	WP_013055005.1	STAS domain-containing protein	-
CR091_RS01150	202201..202557	+	357	WP_013055006.1	STAS domain-containing protein	-
CR091_RS01155	202561..202962	+	402	WP_013055007.1	anti-sigma regulatory factor	-
CR091_RS01160	202976..203986	+	1011	WP_013055008.1	PP2C family protein-serine/threonine phosphatase	-
CR091_RS01165	204046..204378	+	333	WP_013055009.1	anti-sigma factor antagonist	-
CR091_RS01170	204375..204860	+	486	WP_025753516.1	anti-sigma B factor RsbW	-
CR091_RS01175	204826..205620	+	795	WP_028411878.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12991.96 Da
• Isoelectric Point: 4.8781

>T86595 WP_013055004.1 NZ_CP024035:200854-201204 [Priestia aryabhattai]
MVVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVDEALQISVGLIDF

Nucleotide

• Length: 351 bp

>T86595 NZ_CP024035:200854-201204 [Priestia aryabhattai]
TTGGTAGTCAAACGTGGCGACGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGAGGCGTTCGCCCTGT
ACTTGTCATTCAAAATGATATAGGCAATCGTTTTAGCCCAACTGTTATTGTAGCTGCGATCACTGCTCAAATTCAAAAGG
CAAAGTTACCTACGCATGTTGAAATTGATGCAAAGCGCTATGGATTTGAGCGAGATTCAGTTATTTTACTAGAACAAATT
CGTACTATTGATAAACAGAGGCTAACAGACAAGATTACCCATTTAGATGATGAAATGATGGACAGAGTGGATGAAGCCTT
GCAAATCAGTGTAGGACTTATCGACTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10691.22 Da
• Isoelectric Point: 8.5339

>AT86595 WP_013055003.1 NZ_CP024035:200568-200849 [Priestia aryabhattai]
VSEASATTEILVRLPKNLVSELDLMVKQENGNRSDFIHQATKLYLRERKKRHIRESMRRGYMEMAKINLNIASEAFLAEY
EADHTVDRLVSGG

Nucleotide

• Length: 282 bp

>AT86595 NZ_CP024035:200568-200849 [Priestia aryabhattai]
GTGTCTGAAGCGAGCGCAACTACAGAAATCCTAGTAAGATTACCGAAAAATTTAGTATCGGAGCTCGATCTTATGGTTAA
ACAAGAAAATGGCAACCGAAGTGATTTTATTCACCAAGCAACAAAATTGTACCTTCGTGAGCGAAAAAAACGCCATATTC
GCGAATCAATGAGACGTGGATATATGGAGATGGCAAAGATAAACTTAAATATTGCTTCCGAGGCTTTTCTAGCAGAGTAT
GAAGCTGATCATACTGTGGATCGTTTAGTAAGCGGGGGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0B6AMB9