Detailed information of TA system TA086591

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 31142..31683
• Replicon: plasmid pXAC64
• Accession: NZ_CP024030
• Organism: Xanthomonas citri pv. citri strain Xcc49

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CPA10_RS23315
• Protein ID: WP_015462614.1
• Coordinates: 31142..31468 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H8FMA7
• Locus tag: CPA10_RS23320
• Protein ID: WP_003491150.1
• Coordinates: 31465..31683 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPA10_RS23305	26525..28099	+	1575	WP_011052977.1	type IV secretion system DNA-binding domain-containing protein	-
CPA10_RS23310	28113..31088	+	2976	WP_011052978.1	conjugative relaxase	-
CPA10_RS23315	31142..31468	-	327	WP_015462614.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CPA10_RS23320	31465..31683	-	219	WP_003491150.1	antitoxin MazE family protein	Antitoxin
CPA10_RS23325	31820..32206	-	387	WP_003491149.1	hypothetical protein	-
CPA10_RS23330	32283..32702	-	420	WP_011052981.1	hypothetical protein	-
CPA10_RS23335	32828..33706	-	879	WP_011052982.1	transglycosylase SLT domain-containing protein	-
CPA10_RS23340	33717..34739	-	1023	WP_011052983.1	P-type DNA transfer ATPase VirB11	-
CPA10_RS23345	34750..35970	-	1221	WP_011052984.1	TrbI/VirB10 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..64138	64138

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (5-70)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11676.70 Da
• Isoelectric Point: 9.2550

>T86591 WP_015462614.1 NZ_CP024030:c31468-31142 [Xanthomonas citri pv. citri]
MMRGDFVTIAMQGDFGKPRPALVIQADQFDAHTTVTVLPVTSTLVAAPLLRITVHPSTDNGLQKPSQVMVDKAMTVKRDK
VGRAFGRVDADALVEIERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T86591 NZ_CP024030:c31468-31142 [Xanthomonas citri pv. citri]
ATGATGCGGGGTGACTTTGTGACCATCGCCATGCAAGGCGACTTCGGAAAACCAAGGCCCGCTCTGGTGATCCAGGCCGA
CCAGTTTGACGCACACACCACCGTGACGGTGCTGCCGGTGACAAGCACGCTCGTTGCTGCGCCGCTGCTGCGTATTACCG
TTCATCCAAGTACCGACAATGGTTTGCAGAAGCCGTCTCAGGTGATGGTGGACAAGGCCATGACCGTGAAGCGTGACAAG
GTAGGTCGAGCCTTTGGGCGCGTAGACGCTGATGCGTTGGTGGAAATTGAGCGCTGCCTGGCCGTGTTCTTGGGCATCGC
TAAGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8418.54 Da
• Isoelectric Point: 5.6599

>AT86591 WP_003491150.1 NZ_CP024030:c31683-31465 [Xanthomonas citri pv. citri]
MAHVNSRVQKHRDALRMAGLRPVQIWVPDTRRPDFAEECRRQCRLAAQADMADTDMQRFMDEALADMDGWTE

Nucleotide

• Length: 219 bp

>AT86591 NZ_CP024030:c31683-31465 [Xanthomonas citri pv. citri]
ATGGCACACGTCAATTCACGAGTTCAAAAGCACCGCGACGCCCTGCGCATGGCAGGGCTGCGTCCGGTGCAAATTTGGGT
GCCGGACACACGGCGGCCTGACTTCGCCGAGGAATGCCGCCGTCAGTGTCGTCTTGCTGCACAAGCGGACATGGCGGATA
CCGACATGCAGCGCTTCATGGATGAGGCGCTAGCAGACATGGATGGCTGGACGGAATGA

Structures

Antitoxin

• AlphaFold DB: H8FMA7