Detailed information of TA system TA086586

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2720116..2720738
• Replicon: chromosome
• Accession: NZ_CP024025
• Organism: Pseudomonas protegens strain DTU9.1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CLA18_RS12470
• Protein ID: WP_210457091.1
• Coordinates: 2720556..2720738 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CLA18_RS12465
• Protein ID: WP_077933771.1
• Coordinates: 2720116..2720517 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLA18_RS12440 (CLA18_12405)	2716914..2717348	-	435	WP_015634968.1	hypothetical protein	-
CLA18_RS12445 (CLA18_12410)	2717381..2717749	-	369	WP_265578097.1	antibiotic biosynthesis monooxygenase family protein	-
CLA18_RS12450 (CLA18_12415)	2717882..2718316	+	435	WP_019094724.1	MarR family winged helix-turn-helix transcriptional regulator	-
CLA18_RS12455 (CLA18_12420)	2718561..2719268	-	708	WP_265578098.1	DUF1266 domain-containing protein	-
CLA18_RS12460 (CLA18_12425)	2719340..2719612	-	273	WP_227454132.1	DUF5339 family protein	-
CLA18_RS12465 (CLA18_12430)	2720116..2720517	-	402	WP_077933771.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CLA18_RS12470 (CLA18_12435)	2720556..2720738	-	183	WP_210457091.1	type II toxin-antitoxin system HicA family toxin	Toxin
CLA18_RS12475 (CLA18_12440)	2720881..2721579	-	699	WP_041117166.1	arsenical resistance protein ArsH	-
CLA18_RS12480 (CLA18_12445)	2721589..2722059	-	471	WP_152686094.1	arsenate reductase ArsC	-
CLA18_RS12485 (CLA18_12450)	2722091..2723374	-	1284	WP_053157447.1	arsenic transporter	-
CLA18_RS12490 (CLA18_12455)	2723395..2723742	-	348	WP_169894003.1	metalloregulator ArsR/SmtB family transcription factor	-
CLA18_RS12495 (CLA18_12460)	2723973..2724527	+	555	WP_265578099.1	hypothetical protein	-
CLA18_RS12500 (CLA18_12465)	2724772..2725203	-	432	WP_265578100.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6787.97 Da
• Isoelectric Point: 11.5631

>T86586 WP_210457091.1 NZ_CP024025:c2720738-2720556 [Pseudomonas protegens]
VQSRLLIKELEAAGWVLERVTGSHHLFQHPYRPNTVPVSHPKKDLPRGTVRAIRKLAGLL

Nucleotide

• Length: 183 bp

>T86586 NZ_CP024025:c2720738-2720556 [Pseudomonas protegens]
GTGCAAAGCAGACTGTTGATCAAGGAGCTTGAAGCCGCAGGCTGGGTGCTGGAGCGAGTCACCGGCAGCCATCATCTGTT
CCAGCATCCCTACCGGCCTAACACCGTTCCCGTGTCGCATCCGAAGAAGGATCTGCCACGGGGAACGGTCCGGGCCATCA
GGAAACTGGCCGGGCTGCTCTAG

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14514.49 Da
• Isoelectric Point: 5.1222

>AT86586 WP_077933771.1 NZ_CP024025:c2720517-2720116 [Pseudomonas protegens]
VQYPICIEWGDEHTATGIQIPDIPGAVTAGDTFEAAYSAAVEIAHLMLEELARAGQAIPLPSATQTHRAHPDYQGMGWGM
LEIDITPYLGKTEKVNVTLPGYVIQQIDRFVRAHNIKSRSSFLADAALEKLGR

Nucleotide

• Length: 402 bp

>AT86586 NZ_CP024025:c2720517-2720116 [Pseudomonas protegens]
GTGCAATATCCCATCTGCATCGAGTGGGGCGACGAGCACACGGCCACTGGCATCCAGATTCCCGACATTCCCGGCGCAGT
CACCGCCGGCGACACCTTCGAGGCCGCCTACAGCGCCGCCGTCGAGATTGCCCACCTCATGCTCGAAGAGCTGGCCCGCG
CAGGGCAGGCCATTCCCCTGCCCTCTGCCACCCAAACGCACCGCGCCCATCCTGATTACCAGGGGATGGGCTGGGGCATG
CTGGAGATCGACATCACGCCTTACTTGGGCAAGACCGAGAAGGTCAACGTGACCTTGCCGGGGTACGTGATTCAGCAGAT
CGACCGCTTTGTCCGCGCGCACAACATCAAGAGCCGGTCGTCCTTTCTGGCGGATGCGGCGCTGGAAAAGCTCGGGCGCT
AA