Detailed information of TA system TA086585

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 1230759..1231297
• Replicon: chromosome
• Accession: NZ_CP024025
• Organism: Pseudomonas protegens strain DTU9.1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CLA18_RS05500
• Protein ID: WP_220223703.1
• Coordinates: 1231019..1231297 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A7U9L9U0
• Locus tag: CLA18_RS05495
• Protein ID: WP_047283908.1
• Coordinates: 1230759..1231022 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLA18_RS05480 (CLA18_05480)	1226721..1229027	-	2307	WP_210457600.1	TonB-dependent siderophore receptor	-
CLA18_RS05485 (CLA18_05485)	1229234..1229914	+	681	WP_011059221.1	Fe2+-dependent dioxygenase	-
CLA18_RS05490 (CLA18_05490)	1229918..1230703	+	786	WP_077933345.1	tetratricopeptide repeat protein	-
CLA18_RS05495 (CLA18_05495)	1230759..1231022	+	264	WP_047283908.1	ribbon-helix-helix domain-containing protein	Antitoxin
CLA18_RS05500 (CLA18_05500)	1231019..1231297	+	279	WP_220223703.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CLA18_RS05505 (CLA18_05505)	1231368..1232531	-	1164	WP_015634137.1	type III PLP-dependent enzyme	-
CLA18_RS05510 (CLA18_05510)	1233210..1234367	-	1158	WP_015634138.1	ABC transporter ATP-binding protein	-
CLA18_RS05515 (CLA18_05515)	1234364..1235017	-	654	WP_265577836.1	ABC transporter permease	-
CLA18_RS05520 (CLA18_05520)	1235030..1235923	-	894	WP_183001336.1	glycine betaine ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

• Ribbon-helix-helix protein, CopG (7-44)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10724.37 Da
• Isoelectric Point: 10.2944

>T86585 WP_220223703.1 NZ_CP024025:1231019-1231297 [Pseudomonas protegens]
MRQLKWTSKALSDLARLFEFLAQANRSTAARTVQSLVQAPDALLSNPRLGEQLEEFKPRDVRRLLVGHYEMRYEIQGMAL
SILSIWHVREDR

Nucleotide

• Length: 279 bp

>T86585 NZ_CP024025:1231019-1231297 [Pseudomonas protegens]
ATGAGACAGCTGAAATGGACGAGCAAGGCCCTATCCGATCTGGCTCGGCTTTTTGAGTTTCTCGCCCAGGCCAATCGTTC
TACTGCTGCTCGGACCGTGCAATCGCTGGTGCAGGCTCCAGATGCTCTTCTATCTAACCCACGATTGGGAGAGCAGTTGG
AGGAATTCAAGCCGAGGGATGTTCGACGATTGCTGGTGGGGCATTACGAGATGCGTTATGAGATTCAAGGCATGGCCTTG
TCCATCCTGAGTATTTGGCATGTCCGGGAAGATCGTTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9676.08 Da
• Isoelectric Point: 5.6706

>AT86585 WP_047283908.1 NZ_CP024025:1230759-1231022 [Pseudomonas protegens]
MPATKVLTAHIPLDLADKVDQLASRLERSRAWVVKQALVAWVDQEEERRRLTLEALADVDAGRVVDHQSVLAWAQSLSGD
NPLPLPK

Nucleotide

• Length: 264 bp

>AT86585 NZ_CP024025:1230759-1231022 [Pseudomonas protegens]
ATGCCCGCGACAAAAGTACTGACCGCTCACATTCCCTTGGACCTGGCTGACAAGGTCGATCAACTTGCTTCTCGCCTGGA
ACGCTCCCGGGCCTGGGTCGTGAAGCAGGCGTTGGTGGCCTGGGTTGACCAGGAAGAAGAGCGCCGTCGACTGACGCTTG
AGGCCTTGGCAGATGTCGATGCTGGAAGGGTTGTGGATCATCAGTCAGTGTTGGCTTGGGCGCAGAGTCTGTCTGGCGAC
AACCCTCTGCCCTTACCAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9L9U0