Detailed information of TA system TA086583

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 518571..519184
• Replicon: chromosome
• Accession: NZ_CP024025
• Organism: Pseudomonas protegens strain DTU9.1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9LBG9
• Locus tag: CLA18_RS02355
• Protein ID: WP_041115175.1
• Coordinates: 518571..518753 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CLA18_RS02360
• Protein ID: WP_041120520.1
• Coordinates: 518783..519184 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLA18_RS02335 (CLA18_02340)	514309..516270	+	1962	WP_161466434.1	choline transporter BetT	-
CLA18_RS02340 (CLA18_02345)	516280..516537	-	258	WP_015633747.1	hypothetical protein	-
CLA18_RS02345 (CLA18_02350)	516590..517330	-	741	WP_019094920.1	SDR family oxidoreductase	-
CLA18_RS02350 (CLA18_02355)	517463..518353	+	891	WP_265577730.1	LysR family transcriptional regulator	-
CLA18_RS02355 (CLA18_02360)	518571..518753	+	183	WP_041115175.1	type II toxin-antitoxin system HicA family toxin	Toxin
CLA18_RS02360 (CLA18_02365)	518783..519184	+	402	WP_041120520.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CLA18_RS02365 (CLA18_02370)	519197..520243	-	1047	WP_053155900.1	LacI family DNA-binding transcriptional regulator	-
CLA18_RS02370 (CLA18_02375)	520449..522707	+	2259	WP_265577731.1	TonB-dependent receptor	-
CLA18_RS02375 (CLA18_02380)	522851..524179	+	1329	WP_019094918.1	LLM class flavin-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6866.97 Da
• Isoelectric Point: 10.8547

>T86583 WP_041115175.1 NZ_CP024025:518571-518753 [Pseudomonas protegens]
MRSREMISRIEADGWYLVAVKGSHHQYKHPFKPGRVTIRHPDTDLPKGTLNSILKQAGLK

Nucleotide

• Length: 183 bp

>T86583 NZ_CP024025:518571-518753 [Pseudomonas protegens]
ATGCGCAGTCGGGAAATGATCAGCAGGATCGAGGCGGATGGGTGGTACTTGGTGGCCGTCAAGGGCAGTCACCATCAGTA
CAAGCATCCTTTCAAGCCGGGCCGCGTGACCATCAGGCACCCGGATACGGATCTGCCCAAAGGCACGCTCAACAGCATCT
TGAAACAGGCGGGCCTGAAATGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14723.45 Da
• Isoelectric Point: 4.5430

>AT86583 WP_041120520.1 NZ_CP024025:518783-519184 [Pseudomonas protegens]
MKFPVVLHKDADSEYGVTVPDVPGCHSAGPTVSLAFENIKEALSLHYEGLVADGEPLPQVHEIDDHLDNPDYAGGVWGVV
EFDVTPYFGKSVRFNATLPEQLLERIDQRVERDQRYRSRSGFLAAAALRELSA

Nucleotide

• Length: 402 bp

>AT86583 NZ_CP024025:518783-519184 [Pseudomonas protegens]
ATGAAATTCCCGGTGGTTCTGCACAAGGATGCCGACTCCGAATATGGGGTGACAGTCCCGGATGTACCGGGCTGCCATTC
TGCCGGCCCGACGGTTTCCCTGGCGTTCGAGAACATCAAGGAAGCGCTGTCCCTGCACTACGAGGGCCTGGTGGCGGACG
GCGAGCCCTTGCCCCAGGTGCATGAGATTGACGATCACCTGGACAACCCCGACTACGCCGGTGGCGTATGGGGCGTGGTG
GAGTTCGACGTGACGCCCTACTTCGGCAAGTCGGTGCGCTTCAACGCCACCCTGCCGGAGCAACTGCTGGAGCGCATCGA
CCAGAGGGTCGAACGCGATCAGCGCTACCGTTCGCGTTCCGGGTTCCTCGCCGCTGCGGCGCTGCGCGAGCTCTCTGCCT
GA

Structures

Toxin

• AlphaFold DB: A0A7U9LBG9