Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE(toxin)
Location 3226056..3226664 Replicon chromosome
Accession NZ_CP024024
Organism Pseudomonas aeruginosa strain PARM801

Toxin (Protein)


Gene name PfiT Uniprot ID A0A444LUU5
Locus tag BBX44_RS15220 Protein ID WP_019486378.1
Coordinates 3226056..3226403 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID A0A0B0C355
Locus tag BBX44_RS15225 Protein ID WP_003114155.1
Coordinates 3226413..3226664 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BBX44_RS15190 3221415..3221687 + 273 WP_003134390.1 cysteine-rich CWC family protein -
BBX44_RS15195 3221687..3222379 + 693 WP_003124251.1 16S rRNA pseudouridine(516) synthase -
BBX44_RS15200 3222515..3223558 + 1044 WP_121496380.1 L,D-transpeptidase -
BBX44_RS15205 3223638..3224375 + 738 WP_003085453.1 murein L,D-transpeptidase catalytic domain family protein -
BBX44_RS15210 3224827..3225729 + 903 WP_031756465.1 (R)-3-hydroxydecanoyl-ACP:CoA transacylase -
BBX44_RS15220 3226056..3226403 - 348 WP_019486378.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BBX44_RS15225 3226413..3226664 - 252 WP_003114155.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
BBX44_RS15230 3226878..3227861 - 984 WP_121496381.1 tyrosine-type recombinase/integrase -
BBX44_RS15235 3227861..3229153 - 1293 WP_003115206.1 hypothetical protein -
BBX44_RS15240 3229411..3230673 - 1263 WP_121496382.1 hypothetical protein -
BBX44_RS15245 3230675..3231025 - 351 WP_019725830.1 DUF2523 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage catB7 - 3226056..3250323 24267


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-100)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12956.72 Da        Isoelectric Point: 4.4212

>T86578 WP_019486378.1 NZ_CP024024:c3226403-3226056 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEAAVILVLRQKQSVEQQLIRYCLVGPIE

Download         Length: 348 bp

>T86578 NZ_CP024024:c3226403-3226056 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGCGGCAGTGATCTTGGTTCTGCGACAGAAGCAGAGTGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9442.79 Da        Isoelectric Point: 9.4808

>AT86578 WP_003114155.1 NZ_CP024024:c3226664-3226413 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Download         Length: 252 bp

>AT86578 NZ_CP024024:c3226664-3226413 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGACTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A444LUU5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0C355

References