Detailed information of TA system TA086574

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 652702..653744
• Replicon: chromosome
• Accession: NZ_CP024024
• Organism: Pseudomonas aeruginosa strain PARM801

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: BBX44_RS03230
• Protein ID: WP_003153636.1
• Coordinates: 653169..653744 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: BBX44_RS03225
• Protein ID: WP_003050245.1
• Coordinates: 652702..653172 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBX44_RS03190	648078..649496	-	1419	WP_121496457.1	TIGR03752 family integrating conjugative element protein	-
BBX44_RS03195	649486..650412	-	927	WP_121496456.1	TIGR03749 family integrating conjugative element protein	-
BBX44_RS03200	650409..651101	-	693	WP_004350603.1	TIGR03746 family integrating conjugative element protein	-
BBX44_RS03205	651098..651496	-	399	WP_003153632.1	TIGR03750 family conjugal transfer protein	-
BBX44_RS03210	651509..651868	-	360	WP_003153634.1	TIGR03745 family integrating conjugative element membrane protein	-
BBX44_RS03215	651885..652118	-	234	WP_058181971.1	TIGR03758 family integrating conjugative element protein	-
BBX44_RS03220	652115..652498	-	384	WP_033972373.1	RAQPRD family integrative conjugative element protein	-
BBX44_RS03225	652702..653172	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
BBX44_RS03230	653169..653744	+	576	WP_003153636.1	PIN domain-containing protein	Toxin
BBX44_RS03235	653762..654676	+	915	WP_003050256.1	AAA family ATPase	-
BBX44_RS03240	654673..655143	+	471	WP_003153638.1	hypothetical protein	-
BBX44_RS03245	655140..655640	+	501	WP_003090159.1	hypothetical protein	-
BBX44_RS03250	655640..656542	+	903	WP_003153640.1	nucleotidyltransferase domain-containing protein	-
BBX44_RS03255	656581..657306	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	608504..703274	94770

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T86574 WP_003153636.1 NZ_CP024024:653169-653744 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T86574 NZ_CP024024:653169-653744 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAATCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAACGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT86574 WP_003050245.1 NZ_CP024024:652702-653172 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT86574 NZ_CP024024:652702-653172 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68