Detailed information of TA system TA086562

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 191935..192457
• Replicon: plasmid pX39-1
• Accession: NZ_CP023978
• Organism: Klebsiella variicola strain X39

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A9J6S4Z8
• Locus tag: BC497_RS29290
• Protein ID: WP_004187019.1
• Coordinates: 191935..192219 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4ZSR4
• Locus tag: BC497_RS29295
• Protein ID: WP_004187025.1
• Coordinates: 192209..192457 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BC497_RS29265	187134..187640	+	507	WP_038992426.1	Lrp/AsnC family transcriptional regulator	-
BC497_RS29270	187784..188047	-	264	WP_038992428.1	hypothetical protein	-
BC497_RS29275	188149..189117	-	969	WP_162893606.1	IS5 family transposase	-
BC497_RS29280	189412..190437	-	1026	WP_117339745.1	IS110 family transposase	-
BC497_RS29285	190564..191919	+	1356	WP_004187017.1	ISNCY family transposase	-
BC497_RS29290	191935..192219	-	285	WP_004187019.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BC497_RS29295	192209..192457	-	249	WP_004187025.1	plasmid stabilization protein	Antitoxin
BC497_RS29300	192747..193055	-	309	Protein_200	DDE-type integrase/transposase/recombinase	-
BC497_RS29305	193071..194153	-	1083	WP_020803817.1	IS110 family transposase	-
BC497_RS29315	194433..194969	-	537	Protein_202	IS3 family transposase	-
BC497_RS29320	194992..195366	+	375	Protein_203	ATP-binding protein	-
BC497_RS29330	195575..195715	+	141	Protein_204	IS3 family transposase	-
BC497_RS29335	195789..195917	-	129	Protein_205	IS110 family transposase	-
BC497_RS29340	196332..196940	+	609	WP_117339747.1	type 3 fimbria major subunit MrkA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla / mrkA / mrkB / mrkC / mrkF / mrkJ	1..213341	213341
	-	inside	IScluster/Tn	-	-	188149..195366	7217

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11003.72 Da
• Isoelectric Point: 10.5388

>T86562 WP_004187019.1 NZ_CP023978:c192219-191935 [Klebsiella variicola]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

>T86562 NZ_CP023978:c192219-191935 [Klebsiella variicola]
ATGACCTATAAGCTGGCTTTCAACGAATCCGCTCTGAAAGAATGGAAGAAGCTGGGTCATACACTACAGGTACAGTTCAA
AAAGAAACTCAAAGAGCGCCTGGAGAACCCCAGAGTTCCGGCATCACAGCTACATGGACGTAAGGACCAGTATAAAATTA
AGCTACGTGGTGCCGGATATCGCCTTGTCTATCGTGTAGAGGACGACATTGTCACAGTCACTGTTATCGGCGTAGGCAAA
CGCGAAAACGACGACATTTATAATGCAACCCTTAATCGCAATTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9011.24 Da
• Isoelectric Point: 3.9947

>AT86562 WP_004187025.1 NZ_CP023978:c192457-192209 [Klebsiella variicola]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT86562 NZ_CP023978:c192457-192209 [Klebsiella variicola]
ATGGCATTTCAGGTTCTGACCACTACAGCAGCTAGCATTACTGAACTTAAACGTGACCCTATGGGTACTTTCCACGCCGG
GGATGGCGCTCCGGTAGCCATTCTTAATCGTAATGAGCCTGCATTCTACTGTGTGCCACCGGCACTCTATGCCCAGTTGA
TGGAGCTTCTGGAAGATGAAGAGCTGGGACGGATGGCTGATGAGCGAAGCGGAGAAAAGGTTATAGAGGTCAATATTGAT
GACCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZSR4