Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 154364..155274 Replicon plasmid pX39-1
Accession NZ_CP023978
Organism Klebsiella variicola strain X39

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag BC497_RS29105 Protein ID WP_004026354.1
Coordinates 154804..155274 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag BC497_RS29100 Protein ID WP_004181895.1
Coordinates 154364..154807 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BC497_RS29070 149907..150875 + 969 WP_162893602.1 IS5 family transposase -
BC497_RS29075 150962..151420 - 459 WP_032414405.1 type II toxin-antitoxin system YafO family toxin -
BC497_RS29080 151429..151965 - 537 WP_123677973.1 hypothetical protein -
BC497_RS29085 152076..152429 - 354 WP_117339737.1 hypothetical protein -
BC497_RS29090 152409..153377 + 969 WP_162893603.1 IS5 family transposase -
BC497_RS32265 153437..153586 + 150 Protein_159 DNA polymerase V subunit UmuC -
BC497_RS29095 153586..154263 + 678 Protein_160 DUF4113 domain-containing protein -
BC497_RS29100 154364..154807 + 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
BC497_RS29105 154804..155274 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
BC497_RS29110 155384..155644 - 261 WP_004026352.1 hypothetical protein -
BC497_RS29115 156303..157271 - 969 WP_077255787.1 IS5-like element IS903B family transposase -
BC497_RS29120 157293..158462 - 1170 WP_087653879.1 IS481 family transposase -
BC497_RS29125 158721..159332 - 612 Protein_166 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla / mrkA / mrkB / mrkC / mrkF / mrkJ 1..213341 213341
- inside IScluster/Tn - - 148128..170542 22414


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T86560 WP_004026354.1 NZ_CP023978:154804-155274 [Klebsiella variicola]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T86560 NZ_CP023978:154804-155274 [Klebsiella variicola]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACCCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT86560 WP_004181895.1 NZ_CP023978:154364-154807 [Klebsiella variicola]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT86560 NZ_CP023978:154364-154807 [Klebsiella variicola]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References