Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 74527..75170 Replicon plasmid pX39-1
Accession NZ_CP023978
Organism Klebsiella variicola strain X39

Toxin (Protein)


Gene name vagD Uniprot ID A0A155IU92
Locus tag BC497_RS28635 Protein ID WP_000754567.1
Coordinates 74754..75170 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A853H7M9
Locus tag BC497_RS28630 Protein ID WP_001261275.1
Coordinates 74527..74757 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BC497_RS28615 70601..71695 + 1095 Protein_80 restriction endonuclease -
BC497_RS28620 71698..74274 + 2577 WP_087653641.1 EstP -
BC497_RS28630 74527..74757 + 231 WP_001261275.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BC497_RS28635 74754..75170 + 417 WP_000754567.1 type II toxin-antitoxin system VapC family toxin Toxin
BC497_RS28645 75265..76493 + 1229 WP_117339719.1 IS3 family transposase -
BC497_RS28650 76739..77258 - 520 Protein_85 TraI domain-containing protein -
BC497_RS28655 77272..78060 - 789 WP_087653640.1 hypothetical protein -
BC497_RS28660 78060..78525 - 466 Protein_87 hypothetical protein -
BC497_RS32250 79118..79849 + 732 WP_174222428.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla / mrkA / mrkB / mrkC / mrkF / mrkJ 1..213341 213341
- inside IScluster/Tn - - 67155..85738 18583


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15108.61 Da        Isoelectric Point: 8.5403

>T86559 WP_000754567.1 NZ_CP023978:74754-75170 [Klebsiella variicola]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T86559 NZ_CP023978:74754-75170 [Klebsiella variicola]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGTGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCT
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACCGCCATTGC
CGGACACGCCATCGCGGCGGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTTCTGG
AAGACTGGGTTAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8707.72 Da        Isoelectric Point: 4.3410

>AT86559 WP_001261275.1 NZ_CP023978:74527-74757 [Klebsiella variicola]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDP

Download         Length: 231 bp

>AT86559 NZ_CP023978:74527-74757 [Klebsiella variicola]
ATGCGCACAGTATCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGACGAAGGACGCTTTGATCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A155IU92

Antitoxin

Source ID Structure
AlphaFold DB A0A853H7M9

References