Detailed information of TA system TA086557

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 23511..24247
• Replicon: plasmid pX39-1
• Accession: NZ_CP023978
• Organism: Klebsiella variicola strain X39

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: BC497_RS28315
• Protein ID: WP_016240380.1
• Coordinates: 23511..23993 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: BC497_RS28320
• Protein ID: WP_003026799.1
• Coordinates: 23981..24247 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BC497_RS28290	19877..20074	+	198	WP_004152115.1	hypothetical protein	-
BC497_RS28300	20312..21061	+	750	WP_087653615.1	phosphate-starvation-inducible PsiE family protein	-
BC497_RS28305	21048..22010	+	963	WP_004152113.1	M48 family metalloprotease	-
BC497_RS28310	22151..23352	+	1202	Protein_23	IS3 family transposase	-
BC497_RS28315	23511..23993	-	483	WP_016240380.1	GNAT family N-acetyltransferase	Toxin
BC497_RS28320	23981..24247	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
BC497_RS28325	24517..25059	+	543	WP_117339710.1	hypothetical protein	-
BC497_RS28330	25053..25109	-	57	WP_003032489.1	hypothetical protein	-
BC497_RS28335	25227..25937	+	711	WP_087653800.1	PilZ domain-containing protein	-
BC497_RS28340	26055..26639	+	585	WP_047693620.1	LuxR family transcriptional regulator	-
BC497_RS28345	26751..27776	+	1026	WP_117339712.1	IS110 family transposase	-
BC497_RS28350	28071..29015	+	945	WP_162893600.1	IS5-like element IS903B family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla / mrkA / mrkB / mrkC / mrkF / mrkJ	1..213341	213341
	-	inside	IScluster/Tn	-	-	22325..31428	9103

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17323.95 Da
• Isoelectric Point: 9.5822

>T86557 WP_016240380.1 NZ_CP023978:c23993-23511 [Klebsiella variicola]
VGRITAPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T86557 NZ_CP023978:c23993-23511 [Klebsiella variicola]
GTGGGACGTATAACCGCACCCGAGCCGTTATCCAGCTCTCATCAGTTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCATGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGCGGACTCTGTTTCTAAAACTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT86557 WP_003026799.1 NZ_CP023978:c24247-23981 [Klebsiella variicola]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT86557 NZ_CP023978:c24247-23981 [Klebsiella variicola]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66