Detailed information of TA system TA086556

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 197621..198180
• Replicon: chromosome
• Accession: NZ_CP023974
• Organism: Brucella canis strain FDAARGOS_420

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D6LP74
• Locus tag: CRN66_RS06900
• Protein ID: WP_004688321.1
• Coordinates: 197621..197866 (+)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q57DF1
• Locus tag: CRN66_RS06905
• Protein ID: WP_002964097.1
• Coordinates: 197866..198180 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRN66_RS06880	193468..195114	-	1647	WP_004689673.1	hypothetical protein	-
CRN66_RS06885	195249..196022	-	774	WP_006132649.1	hypothetical protein	-
CRN66_RS06895	196850..197488	-	639	Protein_171	tRNA-guanine transglycosylase	-
CRN66_RS06900	197621..197866	+	246	WP_004688321.1	BrnT family toxin	Toxin
CRN66_RS06905	197866..198180	+	315	WP_002964097.1	BrnA antitoxin family protein	Antitoxin
CRN66_RS06915	198528..198698	+	171	WP_002964095.1	DUF1508 domain-containing protein	-
CRN66_RS06920	199046..199757	-	712	Protein_175	porin family protein	-
CRN66_RS06925	199992..200579	-	588	WP_004683738.1	hypothetical protein	-
CRN66_RS06930	200602..200877	-	276	WP_002964092.1	hypothetical protein	-
CRN66_RS06935	200874..201104	-	231	WP_002964091.1	hypothetical protein	-
CRN66_RS06940	201101..201820	-	720	WP_006072696.1	hypothetical protein	-
CRN66_RS06945	201870..202073	+	204	WP_002964088.1	hypothetical protein	-
CRN66_RS06950	202070..202285	+	216	WP_041594673.1	hypothetical protein	-
CRN66_RS06955	202288..202491	+	204	WP_004690812.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wbdA / wboA	190354..204263	13909

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9172.86 Da
• Isoelectric Point: 10.3158

>T86556 WP_004688321.1 NZ_CP023974:197621-197866 [Brucella canis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Nucleotide

• Length: 246 bp

>T86556 NZ_CP023974:197621-197866 [Brucella canis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTCCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11638.42 Da
• Isoelectric Point: 10.3131

>AT86556 WP_002964097.1 NZ_CP023974:197866-198180 [Brucella canis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT86556 NZ_CP023974:197866-198180 [Brucella canis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA