Detailed information of TA system TA086554

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 320584..321158
• Replicon: chromosome
• Accession: NZ_CP023972
• Organism: Pasteurella multocida strain FDAARGOS_385

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: CRN72_RS01615
• Protein ID: WP_096743047.1
• Coordinates: 320874..321158 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CRN72_RS01610
• Protein ID: WP_096743046.1
• Coordinates: 320584..320877 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRN72_RS01590	315758..316849	-	1092	WP_005751571.1	L-ascorbate 6-phosphate lactonase	-
CRN72_RS01595	317199..318989	+	1791	WP_005726754.1	PTS ascorbate-specific subunit IIBC	-
CRN72_RS01600	319034..319501	+	468	WP_005726755.1	PTS sugar transporter subunit IIA	-
CRN72_RS01605	319520..320197	+	678	WP_096743045.1	3-keto-L-gulonate-6-phosphate decarboxylase UlaD	-
CRN72_RS01610	320584..320877	-	294	WP_096743046.1	putative addiction module antidote protein	Antitoxin
CRN72_RS01615	320874..321158	-	285	WP_096743047.1	hypothetical protein	Toxin
CRN72_RS01625	322806..325901	+	3096	WP_138223026.1	STY4851/ECs_5259 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-87)

Antitoxin

• Uncharacterized protein HI1420 (7-92)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10642.30 Da
• Isoelectric Point: 10.1243

>T86554 WP_096743047.1 NZ_CP023972:c321158-320874 [Pasteurella multocida]
VGRLKEVKILSAKSAVLSRINRAMNGNFGDHKSIGNGLYEMRIIKGSGYRVYYGQYGEVTYLLICSGDKSTQKSDIVKAR
ELWKEIKQQEEVKV

Nucleotide

• Length: 285 bp

>T86554 NZ_CP023972:c321158-320874 [Pasteurella multocida]
GTGGGTCGGTTAAAGGAGGTAAAAATTTTATCCGCCAAATCTGCTGTTCTTTCTCGTATTAACAGAGCAATGAATGGTAA
TTTTGGCGATCATAAATCGATTGGAAATGGATTGTATGAGATGAGAATTATAAAAGGTTCGGGTTATAGAGTTTACTATG
GACAATATGGAGAAGTAACTTATTTATTAATTTGTAGTGGCGATAAATCAACACAGAAATCAGACATTGTTAAAGCGAGA
GAGCTGTGGAAAGAAATAAAGCAACAAGAGGAAGTAAAAGTATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10982.83 Da
• Isoelectric Point: 8.0722

>AT86554 WP_096743046.1 NZ_CP023972:c320877-320584 [Pasteurella multocida]
MSKKKIELKPFDIAEHLDSEEMIAAYLSEVLETCDTNEFIFALGHVVRARGMTELAAKTGLRRESLYKTLSYGSKPRFDT
IMKITHSLGIKLVPTHI

Nucleotide

• Length: 294 bp

>AT86554 NZ_CP023972:c320877-320584 [Pasteurella multocida]
ATGAGTAAGAAAAAAATAGAATTAAAACCATTTGATATTGCTGAACATCTAGACAGTGAAGAAATGATTGCGGCATATTT
AAGTGAAGTTTTAGAAACATGTGATACTAATGAATTTATTTTCGCTTTAGGTCATGTTGTAAGAGCAAGAGGAATGACGG
AGTTAGCAGCAAAAACAGGGTTAAGAAGAGAAAGCCTCTATAAAACATTATCATACGGTAGCAAGCCACGTTTTGATACG
ATAATGAAAATTACGCATTCATTAGGTATTAAACTTGTCCCAACGCACATCTAA