Detailed information of TA system TA086546

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 4587956..4588425
• Replicon: chromosome
• Accession: NZ_CP023969
• Organism: Pseudomonas sp. FDAARGOS_380

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CRN80_RS21675
• Protein ID: WP_044273978.1
• Coordinates: 4588150..4588425 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1T1I1G5
• Locus tag: CRN80_RS21670
• Protein ID: WP_044273976.1
• Coordinates: 4587956..4588150 (+)
• Length: 65 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRN80_RS21650	4583940..4584806	+	867	WP_005789024.1	sugar ABC transporter permease	-
CRN80_RS21655	4584817..4585617	+	801	WP_003232361.1	carbohydrate ABC transporter permease	-
CRN80_RS21660	4585629..4585901	+	273	WP_014718862.1	DUF2160 domain-containing protein	-
CRN80_RS21665	4586064..4587806	+	1743	WP_003192009.1	ABC transporter substrate-binding protein	-
CRN80_RS21670	4587956..4588150	+	195	WP_044273976.1	hypothetical protein	Antitoxin
CRN80_RS21675	4588150..4588425	+	276	WP_044273978.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CRN80_RS21680	4588634..4590013	+	1380	WP_098951056.1	iron-containing redox enzyme family protein	-
CRN80_RS21685	4590025..4590969	+	945	WP_098951058.1	class I SAM-dependent methyltransferase	-
CRN80_RS21690	4590976..4592094	+	1119	WP_098952669.1	carboxylate-amine ligase	-
CRN80_RS21695	4592178..4593422	+	1245	WP_098951060.1	glutathione-dependent formaldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-88)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10303.91 Da
• Isoelectric Point: 6.2166

>T86546 WP_044273978.1 NZ_CP023969:4588150-4588425 [Pseudomonas sp. FDAARGOS_380]
MLSVVWLDEAISDLIDIVTFIAAENPGAARRIKTRLEGAPLPLTEHPYLYPNGRVPGTREVVVHPNYVLVYRVTAERIEI
VNVLHARQEYP

Nucleotide

• Length: 276 bp

>T86546 NZ_CP023969:4588150-4588425 [Pseudomonas sp. FDAARGOS_380]
ATGTTGAGTGTTGTTTGGCTTGATGAGGCTATCAGCGACCTGATTGACATCGTTACTTTCATTGCAGCAGAGAATCCTGG
GGCTGCGCGCCGTATCAAGACACGCCTTGAAGGTGCGCCTCTGCCACTGACCGAGCATCCTTACCTGTATCCGAACGGGC
GTGTGCCCGGCACTCGGGAGGTCGTCGTTCACCCCAATTACGTCCTGGTGTATCGAGTAACGGCGGAGCGCATCGAGATA
GTCAACGTGTTGCACGCTCGGCAGGAGTATCCCTGA

Antitoxin

Protein

• Length: 65 a.a.
• Molecular weight: 7112.11 Da
• Isoelectric Point: 5.0509

>AT86546 WP_044273976.1 NZ_CP023969:4587956-4588150 [Pseudomonas sp. FDAARGOS_380]
MGVPLSPIVSEFETEEQASSHDLWFRAKVEKSLANPGPGVPHDEVMARVEAIILEAERKQRVKA

Nucleotide

• Length: 195 bp

>AT86546 NZ_CP023969:4587956-4588150 [Pseudomonas sp. FDAARGOS_380]
ATGGGTGTTCCACTTTCCCCAATTGTTTCCGAATTTGAAACTGAGGAGCAGGCAAGTAGCCATGATCTTTGGTTTCGCGC
CAAAGTCGAAAAAAGCTTGGCCAACCCCGGACCCGGTGTACCCCATGACGAAGTCATGGCGAGGGTTGAGGCGATTATTC
TTGAAGCAGAACGCAAGCAGAGGGTCAAAGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1T1I1G5