Detailed information of TA system TA086531

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3640709..3641327
• Replicon: chromosome
• Accession: NZ_CP023964
• Organism: Yersinia frederiksenii strain FDAARGOS_418

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: Q66DR5
• Locus tag: CRN75_RS16755
• Protein ID: WP_002208622.1
• Coordinates: 3641124..3641327 (+)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: A0A857F2E4
• Locus tag: CRN75_RS16750
• Protein ID: WP_004392643.1
• Coordinates: 3640709..3641077 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRN75_RS16720	3636391..3636651	+	261	WP_004707629.1	type B 50S ribosomal protein L31	-
CRN75_RS16725	3636667..3636810	+	144	WP_002208618.1	type B 50S ribosomal protein L36	-
CRN75_RS16730	3636939..3637817	-	879	WP_004707627.1	metal ABC transporter substrate-binding protein	-
CRN75_RS16735	3637846..3638703	-	858	WP_032910680.1	metal ABC transporter permease	-
CRN75_RS16740	3638700..3639434	-	735	WP_032910697.1	ABC transporter ATP-binding protein	-
CRN75_RS16745	3640251..3640550	+	300	Protein_3225	hypothetical protein	-
CRN75_RS16750	3640709..3641077	+	369	WP_004392643.1	Hha toxicity modulator TomB	Antitoxin
CRN75_RS16755	3641124..3641327	+	204	WP_002208622.1	expression modulating protein YmoA	Toxin
CRN75_RS16760	3642124..3644925	-	2802	WP_138070085.1	inverse autotransporter beta domain-containing protein	-
CRN75_RS16765	3645396..3646307	+	912	WP_004707615.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8063.31 Da
• Isoelectric Point: 6.4573

>T86531 WP_002208622.1 NZ_CP023964:3641124-3641327 [Yersinia frederiksenii]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPPTVWQHVK

Nucleotide

• Length: 204 bp

>T86531 NZ_CP023964:3641124-3641327 [Yersinia frederiksenii]
ATGACAAAAACTGACTACCTGATGCGTTTAAGAAAATGTACGACTATTGATACATTGGAACGTGTAATTGAAAAAAATAA
GTACGAGCTAAGCGATGATGAGCTGGAATTGTTTTACTCAGCTGCAGACCATCGCTTAGCTGAACTCACGATGAATAAAC
TTTATGATAAAATTCCACCTACTGTTTGGCAACATGTGAAATAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14176.95 Da
• Isoelectric Point: 4.5140

>AT86531 WP_004392643.1 NZ_CP023964:3640709-3641077 [Yersinia frederiksenii]
MDEYSPKRHDIAQLKFLCESLYDEGIATLGDSHHGWVNDPTSAVNLQLNDLIEHIASFVMSFKIKYPDGGDLSELVEEYL
DDTYTLFSSYGINDPELRRWQKTKERLFRLFSGEYICTLMKT

Nucleotide

• Length: 369 bp

>AT86531 NZ_CP023964:3640709-3641077 [Yersinia frederiksenii]
ATGGATGAGTACTCACCTAAGCGGCATGACATTGCCCAATTGAAGTTTCTATGTGAGAGTTTGTATGATGAAGGCATTGC
AACCCTTGGTGATAGCCACCATGGTTGGGTAAATGATCCGACATCAGCGGTAAACCTGCAACTCAATGATTTGATCGAGC
ATATAGCTTCATTTGTGATGAGTTTTAAAATAAAATACCCGGATGGCGGCGACCTTTCAGAGTTAGTGGAAGAATATTTA
GACGATACATATACTCTGTTTAGTAGTTATGGGATCAATGATCCTGAATTGCGTCGCTGGCAAAAAACCAAAGAGCGTTT
ATTCAGACTTTTCTCAGGCGAGTACATCTGTACCCTGATGAAAACTTAA

Structures

Toxin

• PDB: 2K5S

Antitoxin

• AlphaFold DB: A0A857F2E4