Detailed information of TA system TA086530

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeV-yfjZ/CbtA-CbeA
• Location: 3547904..3548600
• Replicon: chromosome
• Accession: NZ_CP023964
• Organism: Yersinia frederiksenii strain FDAARGOS_418

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A380PZD3
• Locus tag: CRN75_RS16345
• Protein ID: WP_004712443.1
• Coordinates: 3548265..3548600 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A380PZ29
• Locus tag: CRN75_RS16340
• Protein ID: WP_004712444.1
• Coordinates: 3547904..3548236 (-)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRN75_RS16310	3543696..3544460	-	765	WP_032912052.1	winged helix-turn-helix domain-containing protein	-
CRN75_RS16315	3544801..3544968	-	168	Protein_3140	IS5/IS1182 family transposase	-
CRN75_RS16320	3545064..3546223	+	1160	Protein_3141	IS3 family transposase	-
CRN75_RS16335	3546965..3547819	-	855	WP_004712445.1	DUF4942 domain-containing protein	-
CRN75_RS16340	3547904..3548236	-	333	WP_004712444.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CRN75_RS16345	3548265..3548600	-	336	WP_004712443.1	TA system toxin CbtA family protein	Toxin
CRN75_RS16350	3548656..3548987	-	332	Protein_3145	hypothetical protein	-
CRN75_RS16355	3548981..3549454	-	474	WP_004712441.1	DNA repair protein RadC	-
CRN75_RS16360	3549487..3549924	-	438	WP_004712440.1	antirestriction protein	-
CRN75_RS16365	3550038..3550298	-	261	WP_032912051.1	hypothetical protein	-
CRN75_RS16370	3550295..3550747	-	453	WP_032912049.1	DNA repair protein RadC	-
CRN75_RS16375	3551065..3551331	-	267	WP_032912047.1	hypothetical protein	-
CRN75_RS16380	3551334..3551864	-	531	WP_004712436.1	DUF4234 domain-containing protein	-
CRN75_RS16385	3551914..3552267	-	354	WP_032912046.1	hypothetical protein	-
CRN75_RS16390	3552296..3552985	-	690	WP_004712434.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3513112..3559800	46688
	-	flank	IS/Tn	-	-	3545064..3545375	311

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (5-101)

Antitoxin

• Antitoxin CbeA (10-108)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12227.05 Da
• Isoelectric Point: 7.3980

>T86530 WP_004712443.1 NZ_CP023964:c3548600-3548265 [Yersinia frederiksenii]
MKPRATPPGALPVSAIYQQLLVFLLEHHYGLSLNDTPYHDEQAIAQQIEQGISVGDTINTLVEKYALVRMGRTGFSALAQ
DPMLSKGDIARARQAAGLWRAHFPPTLLYPT

Nucleotide

• Length: 336 bp

>T86530 NZ_CP023964:c3548600-3548265 [Yersinia frederiksenii]
ATGAAACCACGTGCCACTCCACCGGGCGCATTGCCCGTGTCTGCGATTTATCAACAACTGCTCGTTTTCCTGCTGGAGCA
TCACTATGGTCTGAGCCTCAACGATACGCCCTATCACGATGAACAGGCGATTGCGCAGCAGATAGAACAAGGGATTTCAG
TCGGCGACACCATCAACACACTGGTTGAGAAATATGCCTTAGTGCGAATGGGTCGTACCGGGTTCAGCGCACTGGCACAA
GACCCGATGCTGAGTAAGGGGGATATCGCCCGCGCACGCCAGGCTGCGGGTCTGTGGCGTGCTCATTTTCCACCCACTTT
GCTGTACCCGACCTAA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12197.85 Da
• Isoelectric Point: 7.1149

>AT86530 WP_004712444.1 NZ_CP023964:c3548236-3547904 [Yersinia frederiksenii]
MNNNITAASAWGLKRDITPRLGARLVQEGTHLHFLADRAGFVGTFAPDILQTLDTVFPVLIQHLEARLRSGELDQRHQHC
VTVQHAGFTCDADTLGSHGYLYIAVYQKLS

Nucleotide

• Length: 333 bp

>AT86530 NZ_CP023964:c3548236-3547904 [Yersinia frederiksenii]
ATGAATAACAACATTACTGCTGCCTCTGCATGGGGCCTGAAACGTGATATTACGCCACGGTTGGGGGCCCGACTGGTTCA
GGAGGGAACGCATCTGCATTTTCTGGCTGACCGTGCCGGTTTTGTCGGCACATTCGCTCCAGATATCCTCCAAACGTTGG
ATACCGTTTTCCCTGTACTGATTCAACACCTGGAGGCTCGCCTGCGTTCCGGTGAACTCGATCAGCGCCATCAGCACTGC
GTAACTGTCCAGCACGCTGGTTTTACATGCGACGCGGATACGCTGGGCAGTCACGGTTATCTCTATATCGCGGTGTATCA
AAAACTGTCTTGA

Structures

Toxin

• AlphaFold DB: A0A380PZD3

Antitoxin

• AlphaFold DB: A0A380PZ29