Detailed information of TA system TA086508

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 688471..689281
• Replicon: chromosome
• Accession: NZ_CP023963
• Organism: Klebsiella aerogenes strain FDAARGOS_363

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: CRN78_RS03385
• Protein ID: WP_032715534.1
• Coordinates: 688748..689281 (+)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: J2E9Q7
• Locus tag: CRN78_RS03380
• Protein ID: WP_002887278.1
• Coordinates: 688471..688737 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRN78_RS03355	684341..684685	-	345	WP_015368490.1	cation efflux system protein CusF	-
CRN78_RS03360	684703..686088	-	1386	WP_086557053.1	efflux transporter outer membrane subunit	-
CRN78_RS03370	686262..686945	+	684	WP_008807068.1	copper response regulator transcription factor CusR	-
CRN78_RS03375	686935..688392	+	1458	WP_086557054.1	Cu(+)/Ag(+) sensor histidine kinase	-
CRN78_RS03380	688471..688737	+	267	WP_002887278.1	DUF1778 domain-containing protein	Antitoxin
CRN78_RS03385	688748..689281	+	534	WP_032715534.1	GNAT family N-acetyltransferase	Toxin
CRN78_RS03390	689329..690450	-	1122	WP_045364714.1	cupin domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (91-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19908.80 Da
• Isoelectric Point: 5.2753

>T86508 WP_032715534.1 NZ_CP023963:688748-689281 [Klebsiella aerogenes]
MDQLLTIEMIADEFRYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T86508 NZ_CP023963:688748-689281 [Klebsiella aerogenes]
ATGGATCAGCTACTGACGATTGAGATGATTGCCGATGAGTTCCGCTACGACATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAACATCTCAAGCGACAGCACGATGGACAGATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGCTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGCGGCATGCTGCCGTCTAAAACTCAG
CAAAAGAAAATTCCGTATCAAAATGCGCCAAGCGTAACTCTGGGGCGGCTGGCCATTGATAAATCCGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTCGCCCATGCGATGCGAGTGGTGTGGGGGGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCTCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGATGATGAGTCATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9996.46 Da
• Isoelectric Point: 10.1757

>AT86508 WP_002887278.1 NZ_CP023963:688471-688737 [Klebsiella aerogenes]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT86508 NZ_CP023963:688471-688737 [Klebsiella aerogenes]
ATGCCCGCACTCAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACTCAACCGCCAGCACCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGATAA

Structures

Antitoxin

• PDB: 5ZGN
• AlphaFold DB: A0A0H3GLZ1