Detailed information of TA system TA086490

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 68317..68918
• Replicon: plasmid unnamed2
• Accession: NZ_CP023961
• Organism: Escherichia coli strain FDAARGOS_448

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U9YA20
• Locus tag: CRN68_RS28295
• Protein ID: WP_001216045.1
• Coordinates: 68538..68918 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: CRN68_RS28290
• Protein ID: WP_001190712.1
• Coordinates: 68317..68538 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRN68_RS28245	63492..64376	+	885	WP_023356290.1	DUF551 domain-containing protein	-
CRN68_RS28250	64459..64692	+	234	WP_000516537.1	hypothetical protein	-
CRN68_RS28255	64871..65164	+	294	WP_001677496.1	hypothetical protein	-
CRN68_RS28260	65171..65545	+	375	WP_023154376.1	hypothetical protein	-
CRN68_RS28265	65527..66459	+	933	WP_077780118.1	hypothetical protein	-
CRN68_RS28270	66456..66818	+	363	WP_001261544.1	hypothetical protein	-
CRN68_RS29370	66893..67030	-	138	WP_000123562.1	hypothetical protein	-
CRN68_RS28280	67480..67731	+	252	WP_001377386.1	DNA polymerase III subunit theta	-
CRN68_RS28285	67855..68244	+	390	WP_000506726.1	DNA repair protein	-
CRN68_RS28290	68317..68538	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CRN68_RS28295	68538..68918	+	381	WP_001216045.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
CRN68_RS28300	68923..69102	+	180	WP_000113019.1	hypothetical protein	-
CRN68_RS28305	69130..70173	+	1044	WP_000648825.1	DUF968 domain-containing protein	-
CRN68_RS28310	70262..70714	+	453	WP_001369802.1	hypothetical protein	-
CRN68_RS28315	70801..71994	+	1194	WP_000219604.1	terminase	-
CRN68_RS28320	71994..73478	+	1485	WP_000124155.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..96807	96807

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13588.29 Da
• Isoelectric Point: 5.1514

>T86490 WP_001216045.1 NZ_CP023961:68538-68918 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T86490 NZ_CP023961:68538-68918 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT86490 WP_001190712.1 NZ_CP023961:68317-68538 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT86490 NZ_CP023961:68317-68538 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A829CLP7

Antitoxin

• AlphaFold DB: A0A829CJB6