Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4526876..4527530 Replicon chromosome
Accession NZ_CP023960
Organism Escherichia coli strain FDAARGOS_448

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag CRN68_RS24710 Protein ID WP_000244781.1
Coordinates 4527123..4527530 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CRN68_RS24705 Protein ID WP_000354046.1
Coordinates 4526876..4527142 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRN68_RS24685 4522964..4524397 - 1434 WP_001521235.1 6-phospho-beta-glucosidase BglA -
CRN68_RS24690 4524442..4524753 + 312 WP_001182949.1 N(4)-acetylcytidine aminohydrolase -
CRN68_RS24695 4524917..4525576 + 660 WP_000250269.1 hemolysin III family protein -
CRN68_RS24700 4525653..4526633 - 981 WP_000886050.1 tRNA-modifying protein YgfZ -
CRN68_RS24705 4526876..4527142 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CRN68_RS24710 4527123..4527530 + 408 WP_000244781.1 protein YgfX Toxin
CRN68_RS24715 4527570..4528091 - 522 WP_001521233.1 flavodoxin FldB -
CRN68_RS24720 4528203..4529099 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
CRN68_RS24725 4529124..4529834 + 711 WP_001521231.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CRN68_RS24730 4529840..4531573 + 1734 WP_021523238.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T86487 WP_000244781.1 NZ_CP023960:4527123-4527530 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T86487 NZ_CP023960:4527123-4527530 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATCTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT86487 WP_000354046.1 NZ_CP023960:4526876-4527142 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT86487 NZ_CP023960:4526876-4527142 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAGTGTGACGATCCCGATC
TGTTTAACTGGCTGATGAACCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References