Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2883219..2883741 Replicon chromosome
Accession NZ_CP023960
Organism Escherichia coli strain FDAARGOS_448

Toxin (Protein)


Gene name relE Uniprot ID A0A829L6G8
Locus tag CRN68_RS16160 Protein ID WP_001105433.1
Coordinates 2883219..2883509 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SC69
Locus tag CRN68_RS16165 Protein ID WP_000212715.1
Coordinates 2883499..2883741 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRN68_RS16145 2878382..2880037 + 1656 WP_021523332.1 alpha,alpha-phosphotrehalase -
CRN68_RS16150 2880431..2882569 + 2139 WP_000187798.1 anaerobic ribonucleoside-triphosphate reductase -
CRN68_RS16155 2882754..2883218 + 465 WP_001009182.1 anaerobic ribonucleoside-triphosphate reductase-activating protein -
CRN68_RS16160 2883219..2883509 - 291 WP_001105433.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CRN68_RS16165 2883499..2883741 - 243 WP_000212715.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
CRN68_RS16175 2883933..2884319 - 387 WP_001232246.1 cytochrome b562 -
CRN68_RS16180 2884503..2885855 - 1353 WP_001162173.1 metalloprotease PmbA -
CRN68_RS16185 2885949..2886500 + 552 WP_000166270.1 ribosome-associated protein -
CRN68_RS16190 2886652..2888025 - 1374 WP_001522402.1 UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-72)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11367.23 Da        Isoelectric Point: 10.0238

>T86479 WP_001105433.1 NZ_CP023960:c2883509-2883219 [Escherichia coli]
MNYELAFDPRALKEWQKLGVTVREQFKKKLEDVLKRPRNPSAKLRDLPDCYKIKLRTQGYRLVYQVNDKELLVLVIAIGK
RENSAVYEDATKRLDE

Download         Length: 291 bp

>T86479 NZ_CP023960:c2883509-2883219 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9125.56 Da        Isoelectric Point: 5.1369

>AT86479 WP_000212715.1 NZ_CP023960:c2883741-2883499 [Escherichia coli]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAENGRMPVKTLTVSDSEEALLRTVRERLANPQEAIKVRLDEL

Download         Length: 243 bp

>AT86479 NZ_CP023960:c2883741-2883499 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829L6G8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9H4B3

References