86463

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1068609..1069135 Replicon chromosome
Accession NZ_CP023960
Organism Escherichia coli strain FDAARGOS_448

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag CRN68_RS06840 Protein ID WP_000323025.1
Coordinates 1068609..1068896 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag CRN68_RS06845 Protein ID WP_000534858.1
Coordinates 1068896..1069135 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRN68_RS06805 1064602..1064817 - 216 WP_000066484.1 cold shock-like protein CspB -
CRN68_RS06810 1065118..1065330 + 213 WP_000087756.1 cold shock-like protein CspF -
CRN68_RS28660 1065385..1065474 + 90 WP_120795389.1 hypothetical protein -
CRN68_RS06815 1065752..1066504 - 753 WP_001047135.1 antitermination protein -
CRN68_RS06820 1066518..1067567 - 1050 WP_001265199.1 DUF968 domain-containing protein -
CRN68_RS06825 1067569..1067847 - 279 WP_012304870.1 hypothetical protein -
CRN68_RS06830 1067914..1068165 - 252 WP_000980994.1 hypothetical protein -
CRN68_RS06835 1068382..1068537 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
CRN68_RS06840 1068609..1068896 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
CRN68_RS06845 1068896..1069135 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
CRN68_RS06850 1069160..1069465 + 306 WP_001326990.1 hypothetical protein -
CRN68_RS06855 1069668..1070000 + 333 WP_001301033.1 protein FlxA -
CRN68_RS06860 1070437..1071750 - 1314 WP_033559771.1 ISNCY family transposase -
CRN68_RS06870 1072234..1073262 - 1029 WP_001345551.1 DUF4868 domain-containing protein -
CRN68_RS06875 1073259..1073873 - 615 WP_001265627.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1030202..1086360 56158


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T86463 WP_000323025.1 NZ_CP023960:c1068896-1068609 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T86463 NZ_CP023960:c1068896-1068609 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT86463 WP_000534858.1 NZ_CP023960:c1069135-1068896 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT86463 NZ_CP023960:c1069135-1068896 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References