Detailed information of TA system TA086463

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1068609..1069135
• Replicon: chromosome
• Accession: NZ_CP023960
• Organism: Escherichia coli strain FDAARGOS_448

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: CRN68_RS06840
• Protein ID: WP_000323025.1
• Coordinates: 1068609..1068896 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: CRN68_RS06845
• Protein ID: WP_000534858.1
• Coordinates: 1068896..1069135 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRN68_RS06805	1064602..1064817	-	216	WP_000066484.1	cold shock-like protein CspB	-
CRN68_RS06810	1065118..1065330	+	213	WP_000087756.1	cold shock-like protein CspF	-
CRN68_RS28660	1065385..1065474	+	90	WP_120795389.1	hypothetical protein	-
CRN68_RS06815	1065752..1066504	-	753	WP_001047135.1	antitermination protein	-
CRN68_RS06820	1066518..1067567	-	1050	WP_001265199.1	DUF968 domain-containing protein	-
CRN68_RS06825	1067569..1067847	-	279	WP_012304870.1	hypothetical protein	-
CRN68_RS06830	1067914..1068165	-	252	WP_000980994.1	hypothetical protein	-
CRN68_RS06835	1068382..1068537	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
CRN68_RS06840	1068609..1068896	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
CRN68_RS06845	1068896..1069135	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
CRN68_RS06850	1069160..1069465	+	306	WP_001326990.1	hypothetical protein	-
CRN68_RS06855	1069668..1070000	+	333	WP_001301033.1	protein FlxA	-
CRN68_RS06860	1070437..1071750	-	1314	WP_033559771.1	ISNCY family transposase	-
CRN68_RS06870	1072234..1073262	-	1029	WP_001345551.1	DUF4868 domain-containing protein	-
CRN68_RS06875	1073259..1073873	-	615	WP_001265627.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1030202..1086360	56158

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T86463 WP_000323025.1 NZ_CP023960:c1068896-1068609 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T86463 NZ_CP023960:c1068896-1068609 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT86463 WP_000534858.1 NZ_CP023960:c1069135-1068896 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT86463 NZ_CP023960:c1069135-1068896 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8