Detailed information of TA system TA086461

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 885336..885974
• Replicon: chromosome
• Accession: NZ_CP023960
• Organism: Escherichia coli strain FDAARGOS_448

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0D8WF76
• Locus tag: CRN68_RS05900
• Protein ID: WP_001306887.1
• Coordinates: 885336..885512 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CRN68_RS05905
• Protein ID: WP_001270286.1
• Coordinates: 885558..885974 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRN68_RS05875	880392..880850	+	459	WP_000526135.1	IS200/IS605 family transposase	-
CRN68_RS05880	880958..882169	-	1212	WP_023143671.1	BenE family transporter YdcO	-
CRN68_RS05885	882222..882758	+	537	WP_020233877.1	DNA-binding transcriptional regulator SutR	-
CRN68_RS05890	882831..884792	+	1962	WP_024166512.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
CRN68_RS05895	884884..885114	-	231	WP_000494241.1	YncJ family protein	-
CRN68_RS05900	885336..885512	+	177	WP_001306887.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
CRN68_RS05905	885558..885974	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
CRN68_RS05910	886053..887459	+	1407	WP_001523229.1	PLP-dependent aminotransferase family protein	-
CRN68_RS05915	887704..888849	+	1146	WP_020233879.1	ABC transporter substrate-binding protein	-
CRN68_RS05920	888867..889880	+	1014	WP_001523231.1	ABC transporter ATP-binding protein	-
CRN68_RS05925	889881..890822	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	880392..880850	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6768.88 Da
• Isoelectric Point: 11.5336

>T86461 WP_001306887.1 NZ_CP023960:885336-885512 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFRGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T86461 NZ_CP023960:885336-885512 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCGTGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT86461 WP_001270286.1 NZ_CP023960:885558-885974 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT86461 NZ_CP023960:885558-885974 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0D8WF76