Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | kacAT/ElaA-DUF1778 |
| Location | 112814..113617 | Replicon | plasmid unnamed1 |
| Accession | NZ_CP023959 | ||
| Organism | Escherichia coli strain FDAARGOS_448 | ||
Toxin (Protein)
| Gene name | KacT | Uniprot ID | G3CAI8 |
| Locus tag | CRN68_RS01130 | Protein ID | WP_000348883.1 |
| Coordinates | 112814..113344 (-) | Length | 177 a.a. |
Antitoxin (Protein)
| Gene name | KacA | Uniprot ID | G3CAI7 |
| Locus tag | CRN68_RS01135 | Protein ID | WP_001275013.1 |
| Coordinates | 113348..113617 (-) | Length | 90 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CRN68_RS01110 | 108301..108786 | - | 486 | WP_001403958.1 | hypothetical protein | - |
| CRN68_RS01115 | 109177..111798 | + | 2622 | WP_001586230.1 | hypothetical protein | - |
| CRN68_RS01120 | 111997..112227 | + | 231 | WP_001261274.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | - |
| CRN68_RS01125 | 112316..112591 | + | 276 | WP_001403920.1 | type II toxin-antitoxin system VapC family toxin | - |
| CRN68_RS28505 | 112596..112813 | - | 218 | Protein_129 | transposase | - |
| CRN68_RS01130 | 112814..113344 | - | 531 | WP_000348883.1 | GNAT family N-acetyltransferase | Toxin |
| CRN68_RS01135 | 113348..113617 | - | 270 | WP_001275013.1 | DUF1778 domain-containing protein | Antitoxin |
| CRN68_RS01140 | 114505..115494 | + | 990 | WP_123002471.1 | RepB family plasmid replication initiator protein | - |
| CRN68_RS01145 | 115817..116557 | - | 741 | WP_001586233.1 | tyrosine-type recombinase/integrase | - |
| CRN68_RS01150 | 116678..116860 | - | 183 | WP_042004909.1 | hypothetical protein | - |
| CRN68_RS01155 | 116927..117088 | - | 162 | WP_001440647.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| CRN68_RS01160 | 117204..117472 | - | 269 | Protein_136 | type II toxin-antitoxin system ParD family antitoxin | - |
| CRN68_RS01170 | 117768..118508 | - | 741 | WP_029702141.1 | tyrosine-type recombinase/integrase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | blaNDM-5 / sul1 / qacE / aadA2 / dfrA12 / qepA4 / tet(B) / blaTEM-1B | - | 1..128761 | 128761 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 177 a.a. Molecular weight: 20321.20 Da Isoelectric Point: 6.4889
>T86453 WP_000348883.1 NZ_CP023959:c113344-112814 [Escherichia coli]
MDGLRIEIFSEEVEYQLSNFDCGEEYLNTFLTDHLQRQHSSKILRGYLLVTREDRPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCDSL
FYPTKSIEVLFEVNDE
MDGLRIEIFSEEVEYQLSNFDCGEEYLNTFLTDHLQRQHSSKILRGYLLVTREDRPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCDSL
FYPTKSIEVLFEVNDE
Download Length: 531 bp
>T86453 NZ_CP023959:c113344-112814 [Escherichia coli]
ATGGACGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATCAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTCACCGATCATCTGCAACGTCAGCACAGTAGTAAGATTTTACGGGGTTATCTGCTCGTGACGAGAGAGG
ATAGACCACGTGTCATGGGGTACTACACCCTGTCAGGCAGTTGCTTTGAGAAAATACTTCTCCCCAGCAAAACACAACAA
AAGCGTGTGCCCTACAAAAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCATCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AGGCGCTGAACGATAACGCCAAAAAATTCTACCTTCGACTGGGTTTTATACAGTTAAAGGAAGAAAACTGTGATTCGCTC
TTCTACCCGACAAAATCTATTGAAGTATTGTTCGAAGTAAATGACGAATAA
ATGGACGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATCAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTCACCGATCATCTGCAACGTCAGCACAGTAGTAAGATTTTACGGGGTTATCTGCTCGTGACGAGAGAGG
ATAGACCACGTGTCATGGGGTACTACACCCTGTCAGGCAGTTGCTTTGAGAAAATACTTCTCCCCAGCAAAACACAACAA
AAGCGTGTGCCCTACAAAAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCATCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AGGCGCTGAACGATAACGCCAAAAAATTCTACCTTCGACTGGGTTTTATACAGTTAAAGGAAGAAAACTGTGATTCGCTC
TTCTACCCGACAAAATCTATTGAAGTATTGTTCGAAGTAAATGACGAATAA
Antitoxin
Download Length: 90 a.a. Molecular weight: 10229.63 Da Isoelectric Point: 6.4702
>AT86453 WP_001275013.1 NZ_CP023959:c113617-113348 [Escherichia coli]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASARAAEVIDQHRRLLLNEESWNLVMDAITNPPAPNDRLKR
ATNRLRELE
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASARAAEVIDQHRRLLLNEESWNLVMDAITNPPAPNDRLKR
ATNRLRELE
Download Length: 270 bp
>AT86453 NZ_CP023959:c113617-113348 [Escherichia coli]
ATGTCAGCACTGAAGAAGCAGCGCATCGATCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCCATTTCGCAGTTCATGGTCAGTACCGCCTCAGCACGTGCTGCTGAAGTTATTGATCAGCACCGTCGCC
TGCTCCTCAATGAGGAGTCCTGGAATCTGGTTATGGATGCAATCACTAATCCGCCAGCACCAAATGACCGGCTGAAACGC
GCCACTAACCGTCTCCGGGAACTGGAGTAA
ATGTCAGCACTGAAGAAGCAGCGCATCGATCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCCATTTCGCAGTTCATGGTCAGTACCGCCTCAGCACGTGCTGCTGAAGTTATTGATCAGCACCGTCGCC
TGCTCCTCAATGAGGAGTCCTGGAATCTGGTTATGGATGCAATCACTAATCCGCCAGCACCAAATGACCGGCTGAAACGC
GCCACTAACCGTCTCCGGGAACTGGAGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|