Detailed information of TA system TA086452

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 111997..112591
• Replicon: plasmid unnamed1
• Accession: NZ_CP023959
• Organism: Escherichia coli strain FDAARGOS_448

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: CRN68_RS01125
• Protein ID: WP_001403920.1
• Coordinates: 112316..112591 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: G3CAJ1
• Locus tag: CRN68_RS01120
• Protein ID: WP_001261274.1
• Coordinates: 111997..112227 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRN68_RS29150	107500..108272	-	773	Protein_124	hypothetical protein	-
CRN68_RS01110	108301..108786	-	486	WP_001403958.1	hypothetical protein	-
CRN68_RS01115	109177..111798	+	2622	WP_001586230.1	hypothetical protein	-
CRN68_RS01120	111997..112227	+	231	WP_001261274.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CRN68_RS01125	112316..112591	+	276	WP_001403920.1	type II toxin-antitoxin system VapC family toxin	Toxin
CRN68_RS28505	112596..112813	-	218	Protein_129	transposase	-
CRN68_RS01130	112814..113344	-	531	WP_000348883.1	GNAT family N-acetyltransferase	-
CRN68_RS01135	113348..113617	-	270	WP_001275013.1	DUF1778 domain-containing protein	-
CRN68_RS01140	114505..115494	+	990	WP_123002471.1	RepB family plasmid replication initiator protein	-
CRN68_RS01145	115817..116557	-	741	WP_001586233.1	tyrosine-type recombinase/integrase	-
CRN68_RS01150	116678..116860	-	183	WP_042004909.1	hypothetical protein	-
CRN68_RS01155	116927..117088	-	162	WP_001440647.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CRN68_RS01160	117204..117472	-	269	Protein_136	type II toxin-antitoxin system ParD family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaNDM-5 / sul1 / qacE / aadA2 / dfrA12 / qepA4 / tet(B) / blaTEM-1B	-	1..128761	128761

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• SpoVT-AbrB domain (7-45)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 9621.03 Da
• Isoelectric Point: 8.5444

>T86452 WP_001403920.1 NZ_CP023959:112316-112591 [Escherichia coli]
VFSHRIVVSAITYSEMRFGASGPKASPRHVQLVDKFCPCLDAILSWDRAAVDGTTDIKVALRQAGTPIGQNDTAIAGPEA
GRLGKLTSPTL

Nucleotide

• Length: 276 bp

>T86452 NZ_CP023959:112316-112591 [Escherichia coli]
GTGTTTAGTCACCGCATTGTGGTCTCGGCCATTACTTACTCTGAAATGCGCTTCGGTGCCTCCGGTCCGAAGGCCTCCCC
GCGTCACGTGCAACTGGTTGATAAATTCTGCCCGTGCCTCGATGCCATCCTGTCTTGGGACCGCGCCGCGGTGGACGGCA
CCACGGATATTAAAGTGGCACTGCGCCAGGCCGGGACGCCGATCGGCCAGAACGATACGGCGATTGCCGGGCCTGAAGCT
GGAAGACTGGGTAAATTAACATCGCCCACACTCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8856.95 Da
• Isoelectric Point: 4.6286

>AT86452 WP_001261274.1 NZ_CP023959:111997-112227 [Escherichia coli]
MRTVSIFKNGNNRAIHLPRDLDFERVRELEIVREGDSIILRPVQPNWGSFAQLEKADPDFMAERENVVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT86452 NZ_CP023959:111997-112227 [Escherichia coli]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATCCATCTGCCCCGCGATTTGGATTTTGAGCGGGTGAG
AGAGCTGGAGATCGTCCGCGAAGGAGACAGCATCATCCTGCGTCCCGTCCAGCCGAACTGGGGCTCATTCGCGCAGCTCG
AAAAAGCCGATCCGGATTTTATGGCGGAGCGCGAGAATGTTGTCAGCGACGAAGGTCGATTTGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: G3CAJ1