Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 89263..89906 Replicon plasmid unnamed1
Accession NZ_CP023948
Organism Klebsiella pneumoniae strain FDAARGOS_446

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag CRN69_RS28765 Protein ID WP_000754566.1
Coordinates 89263..89679 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag CRN69_RS28770 Protein ID WP_001261276.1
Coordinates 89676..89906 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRN69_RS28750 85323..86291 + 969 WP_004099053.1 IS5-like element IS903B family transposase -
CRN69_RS28755 86409..87686 - 1278 WP_096753237.1 HlyD family secretion protein -
CRN69_RS28760 88345..89222 + 878 Protein_115 restriction endonuclease -
CRN69_RS28765 89263..89679 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
CRN69_RS28770 89676..89906 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CRN69_RS28780 90571..90777 + 207 WP_004153649.1 hypothetical protein -
CRN69_RS28785 90823..91131 + 309 WP_004152336.1 hypothetical protein -
CRN69_RS28790 91159..91488 + 330 WP_004152337.1 hypothetical protein -
CRN69_RS28795 91514..91912 + 399 WP_004171440.1 hypothetical protein -
CRN69_RS28800 91919..92251 + 333 WP_004152339.1 hypothetical protein -
CRN69_RS28805 92251..93033 + 783 WP_004152340.1 site-specific integrase -
CRN69_RS28810 93925..94155 - 231 WP_011977773.1 hypothetical protein -
CRN69_RS28815 94247..94720 - 474 WP_004152341.1 YkgJ family cysteine cluster protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA14 / aac(6')-Ib / qacE / sul1 / rmtC / blaNDM-1 htpB 1..219350 219350
- flank IS/Tn - - 94840..96108 1268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T86427 WP_000754566.1 NZ_CP023948:c89679-89263 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T86427 NZ_CP023948:c89679-89263 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT86427 WP_001261276.1 NZ_CP023948:c89906-89676 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT86427 NZ_CP023948:c89906-89676 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References