Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 33568..34304 Replicon plasmid unnamed2
Accession NZ_CP023947
Organism Klebsiella pneumoniae strain FDAARGOS_446

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag CRN69_RS27445 Protein ID WP_003026803.1
Coordinates 33568..34050 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag CRN69_RS27450 Protein ID WP_003026799.1
Coordinates 34038..34304 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRN69_RS27415 28869..29066 + 198 WP_004152115.1 hypothetical protein -
CRN69_RS27425 29304..30053 + 750 WP_009483782.1 phosphate-starvation-inducible PsiE family protein -
CRN69_RS27430 30040..31002 + 963 WP_004152113.1 M48 family metalloprotease -
CRN69_RS27435 31193..32161 + 969 WP_072198477.1 IS5 family transposase -
CRN69_RS27440 32226..33409 + 1184 Protein_39 IS3 family transposase -
CRN69_RS27445 33568..34050 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
CRN69_RS27450 34038..34304 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
CRN69_RS27455 34480..34734 - 255 WP_004152108.1 hypothetical protein -
CRN69_RS27460 34810..35067 - 258 WP_004152107.1 hypothetical protein -
CRN69_RS27465 35116..35319 - 204 WP_004152106.1 hemolysin expression modulator Hha -
CRN69_RS27470 35353..35721 - 369 WP_004152105.1 hypothetical protein -
CRN69_RS27475 35765..36259 - 495 WP_004152104.1 DNA-binding protein -
CRN69_RS27480 36290..36865 - 576 WP_004152103.1 hypothetical protein -
CRN69_RS27485 36853..37122 - 270 WP_004152102.1 hypothetical protein -
CRN69_RS27490 37480..37830 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
CRN69_RS27495 37880..38242 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr - 1..153385 153385


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T86425 WP_003026803.1 NZ_CP023947:c34050-33568 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T86425 NZ_CP023947:c34050-33568 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT86425 WP_003026799.1 NZ_CP023947:c34304-34038 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT86425 NZ_CP023947:c34304-34038 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References