Detailed information of TA system TA086406

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 4410..4990
• Replicon: plasmid unnamed3
• Accession: NZ_CP023944
• Organism: Klebsiella pneumoniae strain FDAARGOS_444

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: CRN70_RS30425
• Protein ID: WP_071177730.1
• Coordinates: 4410..4724 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: CRN70_RS30430
• Protein ID: WP_000093040.1
• Coordinates: 4712..4990 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRN70_RS30400	354..2318	+	1965	WP_071177727.1	TraM recognition domain-containing protein	-
CRN70_RS30405	2318..3049	+	732	WP_071177728.1	replication-relaxation family protein	-
CRN70_RS30410	3056..3586	+	531	WP_071177729.1	hypothetical protein	-
CRN70_RS30415	3613..3792	-	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
CRN70_RS30420	3818..4246	-	429	WP_001140599.1	hypothetical protein	-
CRN70_RS30425	4410..4724	-	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CRN70_RS30430	4712..4990	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
CRN70_RS31485	5324..5530	-	207	Protein_7	TonB family protein	-
CRN70_RS30440	5527..5898	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
CRN70_RS30445	6172..6417	-	246	WP_032440458.1	hypothetical protein	-
CRN70_RS30450	6744..8429	+	1686	WP_032440457.1	cloacin	-
CRN70_RS30455	8439..8696	+	258	WP_032440455.1	cloacin	-
CRN70_RS30460	8781..8930	+	150	WP_032440454.1	colicin release lysis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10061	10061

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T86406 WP_071177730.1 NZ_CP023944:c4724-4410 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T86406 NZ_CP023944:c4724-4410 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT86406 WP_000093040.1 NZ_CP023944:c4990-4712 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT86406 NZ_CP023944:c4990-4712 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1