Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 70741..71384 Replicon plasmid unnamed2
Accession NZ_CP023942
Organism Klebsiella pneumoniae strain FDAARGOS_444

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag CRN70_RS28345 Protein ID WP_001044770.1
Coordinates 70968..71384 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag CRN70_RS28340 Protein ID WP_001261282.1
Coordinates 70741..70971 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRN70_RS28305 65964..66743 - 780 WP_013214009.1 site-specific integrase -
CRN70_RS28310 66927..67931 - 1005 WP_011977814.1 hypothetical protein -
CRN70_RS28315 67961..68164 - 204 WP_011977813.1 hypothetical protein -
CRN70_RS28320 68210..68731 - 522 WP_013214008.1 hypothetical protein -
CRN70_RS28325 68789..69181 - 393 WP_011977811.1 hypothetical protein -
CRN70_RS28330 69218..70162 - 945 WP_011977810.1 hypothetical protein -
CRN70_RS28335 70323..70784 - 462 WP_014343465.1 hypothetical protein -
CRN70_RS28340 70741..70971 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CRN70_RS28345 70968..71384 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
CRN70_RS28350 71458..73020 + 1563 WP_004206609.1 AAA family ATPase -
CRN70_RS28355 73005..74027 + 1023 WP_000361404.1 helicase UvrD -
CRN70_RS28360 74283..74980 + 698 Protein_99 IS1 family transposase -
CRN70_RS28365 75009..75281 + 273 Protein_100 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid floR / catA2 / blaKPC-2 / rmtB / blaTEM-1B / blaCTX-M-65 - 1..187926 187926


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T86396 WP_001044770.1 NZ_CP023942:70968-71384 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T86396 NZ_CP023942:70968-71384 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT86396 WP_001261282.1 NZ_CP023942:70741-70971 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT86396 NZ_CP023942:70741-70971 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References