Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4110317..4110936 Replicon chromosome
Accession NZ_CP023941
Organism Klebsiella pneumoniae strain FDAARGOS_444

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag CRN70_RS20940 Protein ID WP_002892050.1
Coordinates 4110718..4110936 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag CRN70_RS20935 Protein ID WP_002892066.1
Coordinates 4110317..4110691 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRN70_RS20925 4105469..4106662 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CRN70_RS20930 4106685..4109831 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit -
CRN70_RS20935 4110317..4110691 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
CRN70_RS20940 4110718..4110936 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
CRN70_RS20945 4111095..4111661 + 567 WP_002892030.1 maltose O-acetyltransferase -
CRN70_RS31325 4111633..4111773 - 141 WP_004147370.1 hypothetical protein -
CRN70_RS20950 4111794..4112264 + 471 WP_002892026.1 YlaC family protein -
CRN70_RS20955 4112239..4113690 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
CRN70_RS20960 4113791..4114489 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
CRN70_RS20965 4114486..4114626 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
CRN70_RS20970 4114626..4114889 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T86391 WP_002892050.1 NZ_CP023941:4110718-4110936 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T86391 NZ_CP023941:4110718-4110936 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT86391 WP_002892066.1 NZ_CP023941:4110317-4110691 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT86391 NZ_CP023941:4110317-4110691 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References