Detailed information of TA system TA086373

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 105485..106395
• Replicon: plasmid unnamed1
• Accession: NZ_CP023917
• Organism: Klebsiella pneumoniae strain FDAARGOS_439

Toxin (Protein)

• Gene name: -
• Uniprot ID: R4YBC5
• Locus tag: CRN23_RS28210
• Protein ID: WP_004144067.1
• Coordinates: 105925..106395 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6A8PHD0
• Locus tag: CRN23_RS28205
• Protein ID: WP_023287184.1
• Coordinates: 105485..105928 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRN23_RS28190	100783..101964	+	1182	WP_023287181.1	hypothetical protein	-
CRN23_RS28195	101961..104189	+	2229	WP_023287182.1	Mov34/MPN/PAD-1 family protein	-
CRN23_RS28200	104236..105384	+	1149	Protein_104	translesion error-prone DNA polymerase V subunit UmuC	-
CRN23_RS28205	105485..105928	+	444	WP_023287184.1	DUF2384 domain-containing protein	Antitoxin
CRN23_RS28210	105925..106395	+	471	WP_004144067.1	RES family NAD+ phosphorylase	Toxin
CRN23_RS28215	106482..107450	+	969	WP_000654811.1	IS5 family transposase	-
CRN23_RS28220	107508..107720	-	213	Protein_108	EAL domain-containing protein	-
CRN23_RS28225	107982..108986	-	1005	WP_000427623.1	IS110-like element IS4321 family transposase	-
CRN23_RS28230	109390..110382	-	993	WP_032414406.1	zinc-binding alcohol dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaSHV-12	-	1..164607	164607

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17573.83 Da
• Isoelectric Point: 4.5152

>T86373 WP_004144067.1 NZ_CP023917:105925-106395 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T86373 NZ_CP023917:105925-106395 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACAGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16522.84 Da
• Isoelectric Point: 10.3013

>AT86373 WP_023287184.1 NZ_CP023917:105485-105928 [Klebsiella pneumoniae]
MKTFSLSSSPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT86373 NZ_CP023917:105485-105928 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAAGTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACTGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A384IED8

Antitoxin

• AlphaFold DB: A0A6A8PHD0