Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/ElaA-DUF1778
Location 23853..24604 Replicon plasmid unnamed1
Accession NZ_CP023917
Organism Klebsiella pneumoniae strain FDAARGOS_439

Toxin (Protein)


Gene name ataT Uniprot ID H6U1U8
Locus tag CRN23_RS27730 Protein ID WP_014386536.1
Coordinates 23853..24335 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A071LPN3
Locus tag CRN23_RS27735 Protein ID WP_004902250.1
Coordinates 24326..24604 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRN23_RS27700 20252..20899 - 648 WP_014386537.1 EcsC family protein -
CRN23_RS27705 20926..21681 - 756 WP_004902235.1 DUF2971 domain-containing protein -
CRN23_RS27710 21782..22174 - 393 WP_032442757.1 hypothetical protein -
CRN23_RS27715 22279..22818 - 540 WP_004902239.1 hypothetical protein -
CRN23_RS27730 23853..24335 - 483 WP_014386536.1 GNAT family N-acetyltransferase Toxin
CRN23_RS27735 24326..24604 - 279 WP_004902250.1 DUF1778 domain-containing protein Antitoxin
CRN23_RS27740 24723..24935 - 213 WP_004902255.1 hypothetical protein -
CRN23_RS27745 25043..25384 - 342 WP_004902257.1 hypothetical protein -
CRN23_RS27760 26214..26672 - 459 WP_014386535.1 hypothetical protein -
CRN23_RS27770 26959..27663 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
CRN23_RS27775 27715..28683 + 969 Protein_31 EreB family erythromycin esterase -
CRN23_RS27785 29006..29548 - 543 Protein_32 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaSHV-12 - 1..164607 164607
- flank IS/Tn - - 26959..27663 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(42-144)

Antitoxin

Vibrio phage ICP1, Orf50

(10-86)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17662.45 Da        Isoelectric Point: 8.9130

>T86372 WP_014386536.1 NZ_CP023917:c24335-23853 [Klebsiella pneumoniae]
MGMRAPESLTAEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Download         Length: 483 bp

>T86372 NZ_CP023917:c24335-23853 [Klebsiella pneumoniae]
ATGGGAATGAGAGCGCCTGAATCCCTGACGGCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTCATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGGCCGGGAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATAGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10704.23 Da        Isoelectric Point: 4.5271

>AT86372 WP_004902250.1 NZ_CP023917:c24604-24326 [Klebsiella pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Download         Length: 279 bp

>AT86372 NZ_CP023917:c24604-24326 [Klebsiella pneumoniae]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACGCCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MBI1

Antitoxin

Source ID Structure
AlphaFold DB A0A071LPN3

References