Detailed information of TA system TA086371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 7436..8079
• Replicon: plasmid unnamed2
• Accession: NZ_CP023916
• Organism: Klebsiella pneumoniae strain FDAARGOS_439

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: CRN23_RS26830
• Protein ID: WP_001044770.1
• Coordinates: 7436..7852 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: CRN23_RS26835
• Protein ID: WP_001261282.1
• Coordinates: 7849..8079 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRN23_RS26805	4022..4354	+	333	WP_001393253.1	WbuC family cupin fold metalloprotein	-
CRN23_RS26810	4401..5276	-	876	WP_000239590.1	class A extended-spectrum beta-lactamase CTX-M-15	-
CRN23_RS26815	5532..5903	-	372	Protein_4	transposase	-
CRN23_RS26820	5921..6618	-	698	Protein_5	IS1 family transposase	-
CRN23_RS26825	6652..7362	-	711	WP_004152334.1	ATP-binding protein	-
CRN23_RS26830	7436..7852	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
CRN23_RS26835	7849..8079	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CRN23_RS26840	8036..8497	+	462	WP_072093212.1	hypothetical protein	-
CRN23_RS26845	8732..8938	+	207	WP_004153649.1	hypothetical protein	-
CRN23_RS26850	8984..9292	+	309	WP_004152336.1	hypothetical protein	-
CRN23_RS26855	9320..9649	+	330	WP_004152337.1	hypothetical protein	-
CRN23_RS26860	9675..10073	+	399	WP_004171440.1	hypothetical protein	-
CRN23_RS26865	10080..10412	+	333	WP_004152339.1	hypothetical protein	-
CRN23_RS26870	10412..11194	+	783	WP_004152340.1	site-specific integrase	-
CRN23_RS26880	12408..12881	-	474	WP_004152341.1	YkgJ family cysteine cluster protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / blaTEM-1B	-	1..130348	130348
	-	flank	IS/Tn	-	-	13001..14269	1268

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T86371 WP_001044770.1 NZ_CP023916:c7852-7436 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T86371 NZ_CP023916:c7852-7436 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT86371 WP_001261282.1 NZ_CP023916:c8079-7849 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT86371 NZ_CP023916:c8079-7849 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3