Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 76674..77477 Replicon plasmid unnamed3
Accession NZ_CP023914
Organism Klebsiella pneumoniae strain FDAARGOS_439

Toxin (Protein)


Gene name KacT Uniprot ID W8E895
Locus tag CRN23_RS26540 Protein ID WP_016479963.1
Coordinates 76947..77477 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID W8E6Q5
Locus tag CRN23_RS26535 Protein ID WP_022652312.1
Coordinates 76674..76943 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRN23_RS26515 73160..73312 - 153 Protein_80 IS5/IS1182 family transposase -
CRN23_RS26525 73794..74533 + 740 Protein_81 site-specific integrase -
CRN23_RS26530 74829..75818 - 990 WP_022652311.1 RepB family plasmid replication initiator protein -
CRN23_RS26535 76674..76943 + 270 WP_022652312.1 DUF1778 domain-containing protein Antitoxin
CRN23_RS26540 76947..77477 + 531 WP_016479963.1 GNAT family N-acetyltransferase Toxin
CRN23_RS26550 77609..78625 + 1017 WP_087728544.1 IS5-like element IS5 family transposase -
CRN23_RS26555 78772..79521 - 750 Protein_86 IS3 family transposase -
CRN23_RS26560 79531..79650 - 120 Protein_87 IS30 family transposase -
CRN23_RS29640 79911..80087 - 177 Protein_88 IS91 family transposase -
CRN23_RS26570 80276..81916 - 1641 WP_004201176.1 chaperonin GroEL -
CRN23_RS26575 81972..82262 - 291 WP_004201172.1 co-chaperone GroES -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaNDM-1 / rmtC / sul1 htpB 1..106853 106853
- inside IScluster/Tn - - 55176..79437 24261


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20350.24 Da        Isoelectric Point: 6.4907

>T86370 WP_016479963.1 NZ_CP023914:76947-77477 [Klebsiella pneumoniae]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTREDKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Download         Length: 531 bp

>T86370 NZ_CP023914:76947-77477 [Klebsiella pneumoniae]
ATGGAGGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTTACTGACCATCTGAAACGGCAGCATAACAGTAAGATCTTACGTGGTTATGTTCTCGTGACGAGAGAGG
ATAAACCGCGTGTGATGGGGTACTACACGCTATCAGGAAGTTGCTTTGAGAAAATACTTCTACCCAGCAAAACACAACAA
AAGCGTGTACCCTACAAGAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCACCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AAGCGCTGAACGACAACGCCAAAAAATTCTACCTTCGACTGGGTTTCATTCAGTTAAAGGAAGAAAACTGTAACTCGCTC
TTTTACCCGACAAAATCTATTGAAGAATTGTTCGAAGTAAATGATGAATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10269.70 Da        Isoelectric Point: 5.7252

>AT86370 WP_022652312.1 NZ_CP023914:76674-76943 [Klebsiella pneumoniae]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Download         Length: 270 bp

>AT86370 NZ_CP023914:76674-76943 [Klebsiella pneumoniae]
ATGTCAGCACTGAAAAAGCAGCGCATTGACCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATTTCGCAGTTCATGGTCAGTACCGCCTCAGAACGCGCTGCAGAAGTTATTGACCAGCACCGTCGTC
TGCTCCTCAATGAGGAGTCTTGGAACCTGGTTATGGATGCAATCATTAATCCGCCAGCACCGAATGACCGGCTGAAACGC
GCCGCTAACCGTCTCCGGGAACTGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0L7Q2

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3XDS5

References