Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2956018..2956637 Replicon chromosome
Accession NZ_CP023913
Organism Klebsiella pneumoniae strain FDAARGOS_439

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag CRN23_RS14725 Protein ID WP_002892050.1
Coordinates 2956419..2956637 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag CRN23_RS14720 Protein ID WP_002892066.1
Coordinates 2956018..2956392 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRN23_RS14710 2951170..2952363 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CRN23_RS14715 2952386..2955532 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
CRN23_RS14720 2956018..2956392 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
CRN23_RS14725 2956419..2956637 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
CRN23_RS14730 2956800..2957366 + 567 WP_002892030.1 maltose O-acetyltransferase -
CRN23_RS30050 2957338..2957478 - 141 WP_004147370.1 hypothetical protein -
CRN23_RS14735 2957499..2957969 + 471 WP_020802585.1 YlaC family protein -
CRN23_RS14740 2957944..2959395 - 1452 WP_004183206.1 PLP-dependent aminotransferase family protein -
CRN23_RS14745 2959496..2960194 + 699 WP_023287311.1 GNAT family N-acetyltransferase -
CRN23_RS14750 2960191..2960331 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
CRN23_RS14755 2960331..2960594 - 264 WP_032432663.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T86362 WP_002892050.1 NZ_CP023913:2956419-2956637 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T86362 NZ_CP023913:2956419-2956637 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT86362 WP_002892066.1 NZ_CP023913:2956018-2956392 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT86362 NZ_CP023913:2956018-2956392 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References