Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 463885..464621 Replicon chromosome
Accession NZ_CP023913
Organism Klebsiella pneumoniae strain FDAARGOS_439

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag CRN23_RS02285 Protein ID WP_032433360.1
Coordinates 464139..464621 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag CRN23_RS02280 Protein ID WP_003026799.1
Coordinates 463885..464151 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRN23_RS02255 459531..460670 + 1140 WP_032433368.1 mannitol dehydrogenase -
CRN23_RS02260 460699..461361 + 663 WP_032433366.1 dihydroxyacetone kinase subunit L -
CRN23_RS02265 461345..462349 + 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
CRN23_RS02270 462367..462999 + 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
CRN23_RS02275 463009..463572 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
CRN23_RS02280 463885..464151 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
CRN23_RS02285 464139..464621 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
CRN23_RS02290 464982..465581 - 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
CRN23_RS02295 465794..466738 - 945 WP_077254249.1 fimbrial protein -
CRN23_RS02300 466750..467328 - 579 WP_032432061.1 type 1 fimbrial protein -
CRN23_RS02305 467332..468072 - 741 WP_050597964.1 molecular chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 431830..475564 43734


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T86352 WP_032433360.1 NZ_CP023913:464139-464621 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T86352 NZ_CP023913:464139-464621 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT86352 WP_003026799.1 NZ_CP023913:463885-464151 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT86352 NZ_CP023913:463885-464151 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References